火麻仁油对便秘大鼠肠道炎性反应的改善作用  

Effects of hemp seed oil on intestinal inflammation of constipation rat

作　　者：李寒冰[1] 吴宿慧[1] 李根林[1] 张颜语 吕宁[1] 齐月娟 靳橄 LI Han-bing;WU Su-hui;LI Gen-lin;ZHANG Yan-yu;LYU Ning;QI Yue-juan;JIN Gan(College of Pharmacy,Laboratory of Traditional Chinese Medicine Prevention and Treatment on Senile Diseases,Henan University of Chinese Medicine,Zhengzhou 450046,Chin)

机构地区：[1]河南中医药大学药学院,河南中医药大学老年性疾病中医药防治实验室,郑州450046

出　　处：《中华中医药杂志》2018年第8期3599-3602,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81473435),国家自然科学基金青年项目(No.81503363),河南中医学院科技创新人才支持计划(No.2015XCXRC02),河南省科技厅科学发展计划(No.144300510015),河南省高等学校重点科研项目计划(No.13A360583)

摘　　要：目的:通过研究火麻仁油对便秘大鼠排便情况及结肠炎性反应的变化,结合TLRs信号通路表达,探讨火麻仁油对便秘致肠道炎性反应的影响及作用途径。方法:采用SPF级Wistar大鼠随机分为空白组、模型组、火麻仁油组、阳性对照组。造便秘模型,28d给药结束时对结肠进行病理评分;ELISA法测定血清中TLR4、NF-κB、IL-6、IFN-α变化。结果:与空白组比较,模型组出现明显便秘症状,结肠病理性炎性反应表达增高。与模型组比较,火麻仁油改善便秘症状,降低病理评分,修复病理损伤;改善CHPI评分(P<0.05,P<0.01)。结论:火麻仁油能够缓解便秘状态并减轻肠道炎性损伤,其作用可能与调节TLRs信号通路有关。Objective： To explore the laxative and antiinflammatory actions of hemp seed oil the effects of hemp seed oil on astriction intestinal inflammation and inflammatory factors of constipation model rat were tested. Methods： Wistar rats （SPF grade） were randomly divided into control group, model group, hemp seed oil group and positive control group. The rats of model group were caused constipation by diphenoxylate. Twenty-eight days later, the colon pathological score of the rats were studied by HE staining method, factors （TLR4, NF-κB, IL-6, IFN-α） in serum were determined by ELISA. Results： Compared with the blank group, constipation symptoms developed marked the pathology score of colon raised in model group. Compared with the model group, Hemp seed oil can reduce the damage of intestinal structure, reduce the level of CHPI scores （P〈0.05, P〈0.01）. Conclusion： Hemp seed oil can alleviate constipation, reduce the recovery time of colitis injury in model rats. The functional route of Hemp seed oil relate to regulating the expression of TLRs pathway.

关 键 词：火麻仁油 便秘 肠道炎性反应 TLRs通路 

分 类 号：R285.5[医药卫生—中药学]