雀儿舌头石油醚提取物的化学成分及其抗肿瘤活性研究  

Study on chemical constituents of petroleum ether fraction fromLeptopus chinensis and their antitumor activities

作　　者：魏光璞 孟启[1] 徐淼 于镜坤 刘荐慧 龙跃[1] WEI Guang-pu;MENG Qi;XU Miao;YU Jing-kun;LIU Jian-hui;LONG Yue(College of Chemistry and Molecular Engineering,Zhengzhou University,Zhengzhou,Henan 450001,China)

机构地区：[1]郑州大学化学与分子工程学院,河南郑州450001

出　　处：《时珍国医国药》2018年第4期825-828,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(J1210060);河南省科技攻关计划项目(0624420031);河南省基础研究基金项目(022463001);国家级大学生创新创业训练计划项目(201710459012Z)

摘　　要：目的研究雀儿舌头石油醚提取物的化学成分及其抗肿瘤活性。方法采用硅胶、Sephadex LH-20柱色谱等方法对雀儿舌头石油醚提取物进行分离纯化,根据理化性质和波谱数据鉴定化合物结构,并采用MTT法对部分单体化合物进行体外抗肿瘤活性研究。结果从雀儿舌头中分离得到8个化合物,分别为豆甾醇(1),β-谷甾醇(2),白桦酯酸(3),粉蕊黄杨二醇(4),过氧麦角甾醇(5),二羟基蒲公英烷(6),3α-羟基木栓烷-2-酮(7),乌索酸(8)。化合物5对人前列腺细胞株PC-3、胃癌细胞株MGC-803、人神经母瘤细胞株SK-N-SH的细胞增殖具有良好的抑制作用。结论化合物5、6和8为首次从雀儿舌头中分离得到,化合物5具有一定的抗肿瘤活性。Objective To investigate the chemical constituents of Leptopus chinensis and their antitumor bioactivity. Methods The chemical constituents were isolated by Silica gel column chromatography and Sephadex LH-20 column chromatograph. Their structures were identified by physicochemical properties and spectral analysis. Their cytotoxic activity was studied by MTT. Results Eight compounds were separated and identified as stigmasterol（1）,β-sitosterol（2）,3β-hydroxy-lup-20（29）-ene-28-oic acid（3）,Pachysandiol（4）,Peroxy-ergosterol（5）,dihydroxy-dandelionalkane（6）,3α-hydroxysuberane-2-ketone（7）,ursolic acid（8）. Compound 5 had excellent inhibitory activity on PC-3,MGC-803 and SK-N-SH cells. Conclusion-Compounds 5,6 and 8 are isolated from this plant for the first time,and compound 5 shows the potential antitumor activity.

关 键 词：雀儿舌头 化学成分 抗肿瘤 

分 类 号：R284.2[医药卫生—中药学]