玉米丝黑穗病菌绿荧光蛋白标记菌株的构建与应用  

Application of green fluorescent protein (eGFP)-tagged strains of Sporisorium reilianum

作　　者：邹晓威[1,2] 夏蕾 王娜[1,2] 姜兆远 李莉[1,2] 郑岩 ZOU Xiao-wei;XIA Lei;WANG Na;JIANG Zhao-yuan;LI li;ZHENG Yan(Jilin Academy of Agricultural Sciences,Changchun 130033,China;Key Laboratory of Integrated Pest Management on Crops in Northeast,Ministry of Agriculture,Gongzhuling 136100,China)

机构地区：[1]吉林省农业科学院,长春130033 [2]农业部东北作物有害生物综合治理重点实验室,公主岭136100

出　　处：《植物病理学报》2018年第4期567-571,共5页Acta Phytopathologica Sinica

基　　金：吉林省科技厅发展计划项目(20090710)

摘　　要：玉米丝黑穗病病原菌为丝轴团散黑粉菌（Sporisorium reilianum）,属于担子菌亚门冬孢菌纲黑粉菌目真菌,其引起的玉米丝黑穗病是一种土传病害,在种子时期或经根系侵染寄主[1]。其侵染方式为系统性侵染,病害症状早期不明显,直到该菌株代替寄主植株的雄性或雌性花序时,才出现典型症状[2,3]。PEG-mediated protoplast transformations with three recombinant vectors containing eGFP gene under control of different gene promoters （otef,hsp70,mig2-5-1） were performed in Sporisorium reilianum strains,respectively.All promoters could induce the stable expression of eGFP in the spores,while the signal intensity of fluorescence driven by mig2-5-1 was stronger than the others.Further assaies on the pathogenicity,growth and other characteristics of the eGFP-labeled strains showed the similarity to the wild type.Therefore,the strains labeled with eGFP driven by mig2-5-1 promoter can be used for studying the interaction between Sporisorium reilianum and maize.

关 键 词：玉米丝黑穗病 绿荧光蛋白 黑穗病菌 菌株 应用 标记 侵染方式 担子菌亚门 

分 类 号：S432.44[农业科学—植物病理学]