电针经甲状腺调节治疗乳腺增生大鼠作用机制探讨  

Study on the regulatory mechanism of electroacupuncture based on thyroid pathway for mammary gland hyperplasia rats

作　　者：郭新荣 李宇峰 张姗姗 张迦琅 刘秦浪 程经纬 马晓军 GUO Xinrong;LI Yufeng;ZHANG Shanshan;ZHANG Jialang;L;CHENG Jingwei;MA Xiaojun(College of Acupuncture-Moxibustion and Tuina,Shaanxi University of CM,Xianyang 712046,China)

机构地区：[1]陕西中医药大学针灸推拿学院,咸阳712046

出　　处：《中国针灸》2018年第8期857-863,共7页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81473782;陕西省自然科学基金资助项目:2017 JM 8045;陕西中医药大学自然科学创新基金资助项目:14 XJZR 05

摘　　要：目的:研究甲状腺功能调节在电针治疗乳腺增生(MGH)中的作用,为电针治疗MGH的机制提供新的研究思路,为临床应用提供实验证据。方法:将60只成年雌性SD大鼠随机分空白组、模型组、电针正常治疗组、电针甲切治疗组、电针假手术治疗组,每组12只。除空白组外,余组均复制MGH模型。于模型复制成功后第1天,对电针甲切治疗组进行甲状腺摘除术干预;电针假手术治疗组仅暴露甲状腺,但不进行切除。造模成功后第4天,对电针正常治疗组、电针甲切治疗组、电针假手术治疗组大鼠行电针治疗,余组大鼠不进行电针治疗,捉拿、常规消毒、固定均同上述治疗组。甲组穴为双侧"天宗""肝俞""足三里";乙组穴为双侧"屋翳""合谷""膻中",两组穴位交替使用。同侧"天宗"和"肝俞"穴、同侧"屋翳"和"合谷"穴连接电针仪,每次治疗时共用电针2组,连续波,2 Hz,1 m A,每次20 min,每日1次,5次为一疗程,疗程间休息2 d,共治疗4个疗程。干预后测量各组大鼠乳头高度及直径,采用酶联免疫法(ELISA)检测各组大鼠血清中雌激素(E2)、孕激素(P)含量,采用免疫荧光法和蛋白免疫印迹法(Western-blot)检测各组大鼠乳腺组织中雌激素受体α(ERα)、孕激素受体(PR)含量及蛋白表达。结果:(1)与空白组比较,模型组乳头高度和直径均明显增加(均P<0.01);与模型组比较,电针正常治疗组、电针假手术治疗组乳头高度和直径均明显减小(均P<0.01),电针甲切治疗组与模型组比较也有减小,但差异无统计学意义(均P>0.05)。(2)与空白组比较,模型组血清E2含量升高、P含量下降(均P<0.01);与模型组比较,电针正常治疗组、电针假手术治疗组血清E2均显著下调、血清P含量均显著上调(均P<0.01);与模型组比较,电针甲切治疗组E2、P含量差异均无统计学意义(均P>0.05)。(3)与空白组比较,模型组乳腺组织中ERα含量及蛋白表达升高、PR含量及蛋白�Objective To study the regulating effect of thyroid pathway on electroacupuncture （EA） for mammary gland hyperplasia （MGH） so as to provide new research ideas for the mechanism of EA for MGH and to provide the evidence for clinicaL application. Methods Sixty aduh female SD rats were randomly divided into a blank group, a model group, an EA group, an EA with thyroidectomy group, an EA with sham operation group, 12 rats in each one. Except the blank group, the MGH model was established. Thyroid ablation was performed in the EA with thyroidectomy group, and sham operation was used in the EA with sham operation group, exposing thyroid without excision, 1 day after model establishment. EA was applied in the EA, the EA with thyroidectomy, and the EA with sham operation groups on the 4th day after model establishment, and not used in the other groups, but catching, routine disinfection and fixation were all the same as the above groups. The acupoints in the group A were bilateral ＂Tianzong＂ （SI 11）, ＂Ganshu＂ （BL 18） and ＂Zusanli＂ （ST 36）; and those in the group B were bilateral ＂Wuyi＂ （ST 15）, ＂Hegu＂ （LI 4） and ＂Danzhong＂ （CV 17）. The two groups of points were alternately used. EA, continuous wave, 2 Hz and I mA, was connected at ＂Tianzong＂ （SI 11） and ＂Ganshu＂ （BL 18）, ＂Wuyi＇＂ （ST 15） and ＂Hegu＂ （LI 4） at the same side, 2 pairs EA a lime. 20 rain a time, once a day. All the intervention was given for 4 courses. 5 times as 1course with 2 days between courses. After intervetion. the height and diameter of the rat papilla were measured. Estrogen （E2） and progestational hormone （P） in the serum were detected by enzyme linked immunosorbent assay （ELISA）, and the contents and protein expression of estrogen receptor α （ER α） and progcslerone receptor （PR） in the mammary glands were detected by immunofluoresceuce and Western-blot. Results （1） The height and diameter of papilla in the model group i^lereascd compar

关 键 词：乳腺增生病 电针 甲状腺 雌激素(E2) 孕激素(P) 雌激素受体α(ERα) 孕激素受体(PR) 

分 类 号：R245.97[医药卫生—针灸推拿学]