解淀粉芽孢杆菌B6对拟南芥拮抗病原菌Pst DC3000的作用研究  

Effect of Bacillus amyloliquefaciens B6 on Arabidopsis thaliana antagonistic pathogen Pseudomonas syringae pv tomato DC3000

作　　者：王静[1] 刘娜 赵会君[1] 尚洁[1] WANG Jing;LIU Na;ZHAO Huijun;SHANG Jie(College of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China)

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《北方农业学报》2018年第4期82-86,共5页Journal of Northern Agriculture

基　　金：北方民族大学人才引进启动项目(4400302550);宁夏高等学校科学技术研究项目(NGY2016138)

摘　　要：为研究解淀粉芽孢杆菌B6能否帮助拟南芥抵抗病原菌Pst DC3000侵害,通过皿内拮抗及灌根和喷施盆栽实验,分析解淀粉芽孢杆菌B6对拟南芥拮抗病原菌Pst DC3000的效果。结果表明:解淀粉芽孢杆菌B6能够有效抑制病原菌Pst DC3000生长。观察施加病原菌Pst DC3000后拟南芥叶片,并统计叶片中病原菌数量,发现经B6灌根或喷施预处理的拟南芥,叶片变黄萎蔫时间推后,叶片中病原菌数量显著低于对照组。可见,解淀粉芽孢杆菌B6对拟南芥有较好的拮抗Pst DC3000的作用。这为研究解淀粉芽孢杆菌B6防病机理奠定实验基础。In order to detect whether B. amyloliquefaciens could help plant against the infection of Pst DC3000, using petri dish and pot experiments to analyze the efficiency of B. amyloliquefaciens against the Pst DC3000 infection process in Arabidopsis thaliana. The results showed B. amyloliquefoz.ieus inhibited the growth of＇ Pst DC3000 in petri dish condition. After spayed of Pst DC3000, leaves with pre-treatment, irrigated or sprayed with B. amyloliquefoz.ieus, wilted later than control and the count of Pst DC3000 in leaves were less than control. In conclusion, B. amyloliquefoz.ieus has good inhibition efficieney against Pst DC3000 in petri dish or plants. The study provides experimental basis for further analyze the mechanism of biological control of B. amyloliquefacieus.

关 键 词：解淀粉芽孢杆菌 拟南芥 PST DC3000 

分 类 号：S482.292[农业科学—农药学]