温针灸联合骨髓间充质干细胞移植对兔膝骨关节炎的影响  

Effects of Warming Needle Moxibustion Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on Knee Osteoarthritis in Rabbits

作　　者：刘君伟[1] 张艳玲[2] 闻兵[1] 武永利[2,3] LIU Junwei;ZHANG Yanling;WEN Bing;WU Yongli(Ningxia Medcial University,Yinchuan 750004;Orthopedics and Traumatology Department,the General Hospital of Ningxia Medcial University,Yinchuan 750004;Key Laboratory of Hui Ethnic Medicine Modernization,Ministry of Education of Ningxia Medcial University,Yinchuan 750004)

机构地区：[1]宁夏医科大学中医学院,银川750004 [2]宁夏医科大学总医院中医骨伤科,银川750004 [3]宁夏医科大学回医药现代化教育部重点实验室,银川750004

出　　处：《宁夏医科大学学报》2018年第5期517-522,532,共7页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81641148);2016年自治区科技创新领军人才项目(KJT201610)

摘　　要：目的探讨温针灸联合骨髓间充质干细胞(BMSCs)移植对兔膝骨关节炎(KOA)的作用。方法采用右后肢伸直位固定法制备兔KOA中期软骨退变模型,雄性新西兰大耳兔50只,随机分成5组:空白组、模型组、温针灸组、BMSCs组、温针灸联合BMSCs组,每组10只。采用彭太平法观察兔KOA软骨形态学变化;采用苏木精-伊红染色法对兔KOA关节软骨组织进行病理观察,依据Mankin法予以评估;采用TUNEL法观察软骨细胞凋亡情况;免疫组化法观测兔膝关节软骨基质中Ⅱ型胶原的表达。结果与空白组相比,模型组膝关节软骨损伤程度、Mankin评分和软骨细胞凋亡指数均升高,Ⅱ型胶原蛋白表达降低(P<0.05);经温针灸联合BMSCs移植干预后,兔KOA软骨组织损伤程度、Mankin评分与软骨细胞凋亡指数降低,Ⅱ型胶原蛋白表达升高(P<0.05),并且上述指标均优于温针灸组和BMSCs组(P<0.05)。结论温针灸联合BMSCs移植可以对兔KOA发挥保护作用,并且可以作为临床治疗KOA的潜在方法。Objective To investigate the impact of warming needle moxibustion combined with bone marrow mesenchymal stem cell（BMSCs）transplantation in rabbits experimental model of knee osteoarthritis（KOA）.Methods The rabbit model of medium-term KOA cartilage degeneration was prepared by the method of right hind limb extension. The 50 male New Zealand big ears rabbits were randomly divided into 5 groups： blank group,model group,warm acupuncture group,BMSCs group,warm acupuncture and moxibustion combined with BMSCs group,with 10 rats in each group. The morphological changes of rabbit KOA cartilage were observed by pomtaiping,and the cartilage tissue of rabbit KOA joint was observed by hematoxylin and eosin staining,and evaluated by Mankin method. The apoptosis of cartilage cells was examined by TUNEL method,and the expression of collagen Ⅱ in the cartilage matrix of rabbit knee was observed by immunohistochemistry. Results Compared with the blank group,the degree of cartilage injury, Mankin score and apoptosis index of the knee joint in the model group increased and the expression of collagen Ⅱ decreased（P〈0.05）,and the degree of injury of cartilage tissue,the Mankin score and the number of apoptotic cartilage cells decreased,and the expression of collagen Ⅱ increased（P〈0.05）by the combination of warm acupuncture and BMSCs transplantation（P〈0.05）,and the above indexes were better than those of Wen acupuncture group and BMSCs group（P〈0.05）. Conclusion Warming needle moxibustion combined with BMSCs transplantation can protect KOA and can be used as a potential method for clinical treatment of KOA.

关 键 词：膝骨关节炎 温针灸 骨髓间充质干细胞 Ⅱ型胶原 

分 类 号：R244.9[医药卫生—针灸推拿学]