抗氧化剂对二氧化硫诱导的大鼠心脏线粒体损伤的缓解作用  

Antioxidants alleviated sulfur dioxide-induced mitochondrial damage in rat hearts

作　　者：夏瑾[1] 秦国华[1,2] 桑楠 XIA Jin;QIN Guo-hua;SANG Nan(College of Environmental Science and Resources,Shanxi University,Taiyuan 030006,China;Guangzhou Key Laboratory of Environmental Exposure and Health,School of Environment,Jinan University,Guangzhou 510632,China)

机构地区：[1]山西大学环境与资源学院,山西太原030006 [2]暨南大学环境学院,广州市环境暴露与健康重点实验室,广东广州510632

出　　处：《中国环境科学》2018年第8期3129-3134,共6页China Environmental Science

基　　金：国家自然科学基金资助项目(21777091); 广州市环境暴露与健康重点实验室开放基金资助项目(GZKLEEH201612); 环境化学与生态毒理学国家重点实验室开放基金资助项目(KF2016-17)

摘　　要：采用Wistar大鼠作为模型进行整体动物染毒,SO_2组动式吸入SO_2(7mg/m^3)28d,每天4h;SO_2+NALC(N-乙酰半胱氨酸)组吸入同样条件的SO_2,且自SO_2染毒之日起隔天腹腔注射50mg/kg b.w.NALC,对照组吸入新鲜空气并注射生理盐水.采用荧光定量PCR技术检测心脏组织中氧化磷酸化复合体亚基CO1和ATP6以及核转录因子PGC1-α、NRF1、TFAM的mRNA转录水平;并采用Western blot技术检测3种线粒体调控基因的蛋白表达.结果发现,在吸入SO_2后,核转录因子PGC1-α、NRF1、TFAM的mRNA转录和蛋白表达水平显著降低,并且2种亚基CO1和ATP6的mRNA转录水平也显著下降,而抗氧化剂NALC处理能够明显缓解3种核转录因子及2种复合体亚基表达水平的下降.提示SO_2暴露通过下调PGC1-α、NRF1、TFAM表达来影响线粒体DNA的转录,干扰氧化磷酸化重要组分的合成,该过程发生机制可能与自由基的产生相关,而抗氧化剂的使用可有效缓解SO_2诱导的心脏线粒体损伤作用.Male Wistar rats were exposed to SO2（7 mg/m^3） for 28 days, 4 hours per day in SO2 group. Rats in SO2＋NALC group were exposed to SO2 and NALC（50 mg/kg b.w., i.p.） every other day, which was dissolved in saline. Rats in control group were exposed to filtered air and saline. The m RNA levels of complexes IV and V subunits（CO1ATP6） and three mitochondrial transcript factors（PGC1-α, NRF1, TFAM） were analyzed by real-time RT-PCR after exposure. And the protein levels of the above three mitochondrial transcript factors were detected by Western blot. The results showed that the m RNA and protein expression levels of PGC1-α, NRF1 and TFAM were decreased significantly after SO2 inhalation, combined with the down-regulations of CO1ATP6 on m RNA levels. But NALC could alleviate the depressions of these genes. It indicated that SO2 exposure could impact the transcription of mitochondria DNA through PGC1-α-NRF1-TFAM down-regulation, interfere the synthesis of important components of oxidative phosphorylation. The mechanism might be related to the production of free radicals. Antioxidants could be used to alleviate sulfur dioxide-induced mitochondrial damage in rat hearts.

关 键 词：SO2 抗氧化剂 线粒体 氧化磷酸化 

分 类 号：X503.22[环境科学与工程—环境工程]