分散液相微萃取-液质联用法快速测定党参中有机磷农药残留  

Rapid Determination of Organophosphorus Pesticides Residues in Codonopsis Radix by Disperse Liquid-liquid Microextraction Coupled with Liquid Chromatography-Mass Spectrometry

作　　者：周卿 张艳 杨鑫 ZHOU Qing;ZHANG Yan;YANG Xin(Teaching and Research Section of Pharmaceutical Analysis,College of Pharmacy,Zunyi Medical University,Zunyi 563003,Guizhou,Chin)

机构地区：[1]遵义医学院药学院药物分析教研室,贵州遵义563003

出　　处：《湖北农业科学》2018年第15期81-83,87,共4页Hubei Agricultural Sciences

基　　金：贵州省中医药管理局项目(QZYY2012-D406)

摘　　要：采用分散液相微萃取-液相色谱串联质谱仪,建立党参(Codonopsis Radix)中8种有机磷农药的快速测定方法。以乙睛为分散剂,氯仿为萃取剂富集党参中8种有机磷类农药,采用+MRM监测模式检测,外标法定量。结果表明,8种有机磷农药在各自浓度范围内线性关系良好,相关系数均大于0.990 0;高、中、低3个浓度的加标回收率范围在76.81%~110.25%;各农药的方法检测限范围为0.006~1.300μg/kg,表明该方法可用于党参中多种有机磷农药残留量的快速测定。To establish disperse liquid-liquid microextraction coupled with liquid chromatography-mass spectrometry（DLLME-LCMS） method for determination of eight organophosphorus pesticides（dimethoate,dichlorvos,malathion,parathion,phorate,diazinon,ethion,chlorpyriphos） in Codonopsis radix. The eight organophosphorus pesticides in Codonopsis radix which was extracted by trichlormethane,and the acetonitrile was dispersant,then analysed by using mass spectrometric ditection in multiple reaction monitoring（MRM） mode under the optimal chromatographic conditions. The external standard method was adopted for quantitative analysis. The eight organophosphorus pesticides have a good linear relation respectively（r ﹥ 0.999 0）. The recoveries at three spiked levels of eight organophosphorus pesticides ranged from 76.81% to 110.25%,the detection limits were 0.006~1.300μg/kg. The method can be applied in detection of the organophosphorus pesticides residues analysis in Codonopsis radix.

关 键 词：党参(Codonopsis Radix) 有机磷类农药 分散液相微萃取 液质联用法 

分 类 号：O657.72[理学—分析化学] S567.239[理学—化学]