核桃根际溶磷菌的筛选  

Selection of Rhizosphere Phosphate-solubilizing Bacteria for Juglans regia

作　　者：李诗娟[1] 宋祖文 王丽[1] 黄仲华 盛玉珍[1] 金开铭 张玲 徐磊 LI Shi-juan;SONG Zu-wen;WANG Li;HUANG Zhong-hua;SHENG Yu-zhen;JIN Kai-ming;ZHANG Ling;XU Lei(Sichuan Academy of forestry,Chengdu 610081,China;Forestry Bureau of Xide County,Xide 616750,China;Sichuan Forestrg Propagonda Center Chengdu 61000,China)

机构地区：[1]四川省林业科学院,四川成都610081 [2]四川林业宣传中心,四川成都610000 [3]喜德县林业局,四川喜德616750

出　　处：《四川林业科技》2018年第4期22-25,53,共5页Journal of Sichuan Forestry Science and Technology

基　　金：四川林业厅项目

摘　　要：为获得高效的溶磷菌,用于菌肥生产。通过Pikovskava无机解磷培养基从核桃土壤里筛选出溶磷菌,通过液体发酵试验确定其溶磷活力,利用分子生物学方法对较高活力的溶磷菌进行分子鉴定;通过单因素试验,比较该溶磷菌与两种菌肥溶磷菌在不同发酵液配方及发酵条件下的发酵活力。分离得到的高效溶磷菌为芽孢菌属,适宜初始p H值范围为5~9,适宜温度范围为16℃~40℃,适宜氮源有硫酸铵、氯化铵和草酸铵、蛋白胨,适宜碳源有草酸铵、蔗糖、甘油;高浓度Fe^(3+)、Mn^(2+)对其活力有一定的促进作用,而Cu^(2+)、Ni^(2+)、Zn^(2+)会抑制其解磷。在不同发酵液配方及发酵条件下,该溶磷菌溶磷能力优于菌肥2溶磷菌;在不同发酵条件下,该溶磷菌溶磷能力稳定性优于菌肥1溶磷菌。该溶磷菌添加到菌肥中有一定的市场潜力。It aimed to gain highly efficient phosphate-solubilizing microorganisms.Phosphate-solubilizing bacteria were isolated in advantage of Pikovskava phosphate dissolving medium.Liquid fermentation experiments showed the phosphate-solubilizing activity,molecular biological methods identified the microbial functional community with higher activity.By single factor test,the vitality of different strains were compared under different formulas and the fermentation conditions of culture medium. Bacillus sp.was the phosphate-solubilizing bacterium with higher efficiency.The optimum medium was as follows：suitable initial pH of 5~9,optimum temperature range of 16 ℃~40 ℃,ammonium sulphate,ammonium chloride and ammonium oxalate,peptone for suitable nitrogen source,ammonium oxalate,sucrose,glycerol for suitable carbon source.High concentration of Fe^3＋ and Mn^2＋ had a certain role in promoting its vitality,but Cu^2＋ ,Ni^2＋ and Zn^2＋ would hold the phosphate solubilizing.In different formulations and fermentation conditions,Its phosphate solubilizing ability was better than that of microbial fertilizer 2.In different fermentation conditions,the stability of phosphate solubilizing ability was better than that of microbial fertilizer 1.It would give a certain market potential for the phosphate-solubilizing bacteria added to the fertilizer.

关 键 词：溶磷菌 溶磷能力 优化 

分 类 号：S664.1[农业科学—果树学]