猪乙型脑炎灭活疫苗安全性与免疫效力的研究  

Study on the safety and immune efficacy of inactivtated Swine Japanese Encephalitis Vaccine(strain GD)

作　　者：赖月辉 牛晓芸 牛贝贝 朱炜斌 李嘉爱 黄秋雪 齐冬梅 LAI Yuehui;NIU Xiaoyun;NIU Beibei;ZHU Weibin;LI Jiaai;HUANG Qiuxue;QI Dongmei(Guangdong Winsun bio-pharmaceutical co.,ltd Guangzhou 511356)

机构地区：[1]广东永顺生物制药股份有限公司,广东广州511356

出　　处：《广东畜牧兽医科技》2018年第4期43-46,共4页Guangdong Journal of Animal and Veterinary Science

摘　　要：本研究对猪乙型脑炎灭活疫苗(GD株)的安全性和免疫效力进行了评估。用体重为8~10 g SPF鼠进行5批疫苗的安全性比较,结果:5批猪乙型脑炎灭活疫苗(GD株)的安检鼠精神、食欲正常,没有出现不良反应,表明猪乙型脑炎灭活疫苗对SPF鼠是安全的。用30日龄的健康易感仔猪进行疫苗的安全性比较,结果:5批猪乙型脑炎灭活疫苗(GD株)的安检猪体温、精神、食欲正常,没有出现不良反应,安检猪的平均增重与对照猪的平均增重无显著差异,猪的安全性试验结果表明猪乙型脑炎灭活疫苗对猪安全。用体重为8~10 g SPF鼠对猪乙型脑炎灭活疫苗进行免疫保护性试验,SPF鼠注射疫苗后21日,进行猪乙型脑炎病毒(GD株)(病毒含量为106.0TCID50/mL)强毒攻击,结果:攻毒后,5批猪乙型脑炎灭活疫苗(GD株)免疫小鼠的精神、食欲正常,全部健活,免疫组保护率10/10,对照组小鼠全部发病、死亡率10/10。小鼠的免疫攻毒试验结果表明猪乙型脑炎灭活疫苗(GD株)可以刺激机体产生良好的免疫应答,对小鼠的保护率高。In this study,we evaluated the safety and immune efficacy of Swine Japanese Encephalitis vaccine（strain GD）,inactivtated. Five batches of vaccine were used to estimate the vaccine on specific-pathogen-free（SPF）mouse and healthy piglet. SPF mouse inoculated with five batches of vaccine were orthophrenia,eusitia and with no adverse effect, respectively. Healthy piglets of 30-day-old inoculated with 5 bathces of the vaccine were normothermia,orthophrenia,eusitia and with no adverse effect,respectively. No significant different of average weight gain was found between vaccinated piglets and unvaccinated piglets. Results indicate that this vaccine was safe to SPF mouse and healthy piglet. In the immune efficacy test,SPF mouse were challenged with virulent JEV 21 days after inoculation,mouse inoculated with five batches of vaccine were 100 % survived,while the mortality of unvaccinated mouse was as high as 100 %. The results of the mouse＇s immune challenge test showed that inactivtated Swine Japanese Encephalitis vaccine（strain GD）could stimulate the body to produce a good immune response,which had a high protective rate for the mice.

关 键 词：猪乙型脑炎灭活疫苗 安全性 免疫效力 

分 类 号：S852.52[农业科学—基础兽医学]