葛根素促进成骨细胞复合β-磷酸三钙的成骨  

Puerarin promotes osteogenesis of osteoblasts compounded with beta-tertiary calcium phosphate

作　　者：常再平 杨海龙 张明宇[3] Chang Zai-ping;Yang Hai-long;Zhang Ming-yu(Department of Orthopedics,the Second Hospital of Weinan Weinan 714000,Shaanxi Province,China;Department of Orthopedics,Weinan Hospital,Weinan 714000,Shaanxi Province,China;Department of Sports Medicine,Xi＇an Honghui Hospital,Xi＇an Jiaotong University,Xi＇an 710054,Shaanxi Province,China)

机构地区：[1]渭南市第二医院骨科,陕西省渭南市714000 [2]渭南医院骨科,陕西省渭南市714000 [3]西安交通大学附属红会医院运动医学科,陕西省西安市710054

出　　处：《中国组织工程研究》2018年第28期4447-4451,共5页Chinese Journal of Tissue Engineering Research

基　　金：陕西省自然科学基金项目(2016JM8138);陕西省卫计委卫生科研项目(2014D51)~~

摘　　要：背景:葛根素提取自豆科植物野葛的根部,根据其化学结构可归类为异黄酮类化合物,据报道异黄酮具有促进成骨细胞增殖分化功能。目的:观察不同浓度葛根素对β-磷酸三钙成骨细胞复合物的影响,探讨葛根素对体内成骨作用的影响。方法:(1)取新生兔颅盖骨进行成骨细胞培养,应用碱性磷酸酶试剂盒及茜素红染色方法观察不同浓度葛根素对体外培养成骨细胞碱性磷酸酶活性及矿化结节形成影响;(2)选促进成骨细胞活性最佳浓度葛根素组作为A组,无葛根素培养的细胞为B组,2组培养的成骨细胞复合于β-磷酸三钙,使用MTT法检测支架材料中成骨细胞活性;(3)将2组细胞支架复合物植入兔背阔肌中,通过组织学方法分析葛根素在体内对新骨生成的作用。结果与结论:葛根素可提高成骨细胞碱性磷酸酶活性及矿化结节形成数量,1×10^(-6) mol/L葛根素能明显提高支架材料中成骨细胞活性,将1×10^(-6) mol/L葛根素干预的细胞制成的细胞支架复合物植入兔背阔肌后,新骨生成量明显增加。提示葛根素具有促进β-磷酸三钙成骨细胞复合物体内新骨生成作用,可作为促进成骨治疗的选择药物。BACKGROUND： Puerarin is the major bioactive ingredient isolated from the root of the Pueraria lobata（Willd.） Ohwi, which is a kind of isoflavones. Isoflavones has been shown to promote the proliferation and differentiation of osteoblasts. OBJECTIVE： To observe the effect of puerarin at different concentrations on the osteogenic activity ofβ-tertiary calcium phosphate（β-TCP） complex, and to investigate the effect of puerarin on osteogenesis in vivo. METHODS： Osteoblasts isolated from calvarial of newborn rabbits were cultured in vitro with different concentrations of puerarin. The alkaline phosphotase activity and mineral node formation were determined by alkaline phosphatase kit and alizarin red staining. The osteoblasts cultured with the optimal concentration of puerarin（group A） or without puerarin（group B） were seeded on β-TCP, respectively. Osteoblast activity was determined using MTT assay. Scaffold complexes in each group were implanted into the rabbit dorsal muscles. Osteogenesis was analyzed by histological analysis. RESULTS AND CONCLUSION： The alkaline phosphatase activity and mineral nodules were significantly increased in osteoblasts cultured with puerarin, and 1×10^（-6） mol/L puerarin could significantly incresase increase the osteoblast activity in the scaffold. After the β-TCP composite scaffolds cultured with 1×10^（-6） mol/L puerarin were implanted into the rabbit dorsal muscles, the newly formed bone was significantly increased. In summary, puerarin enhances the osteogenic effect of β-TCP osteoblast complex in vivo, which can be used to promote osteogenesis.

关 键 词：成骨细胞 磷酸钙类 组织工程 葛根素 Β-磷酸三钙 成骨 促进 复合 碱性磷酸酶 矿化结节 

分 类 号：R318[医药卫生—生物医学工程]