早期股骨髋臼撞击症模型兔的建立及其形态学特征  

Establishment of a rabbit model of early femoroacetabular impingement and its morphological characteristics

作　　者：熊奡[1] 栾昕宇 林健静 康斌[1] 曾晖[1] Xiong Ao;Luan Xin-yu;Lin Jian-jing;Kang Bin;Zeng Hui(Department of Bone and Joint,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong Province,China)

机构地区：[1]北京大学深圳医院骨关节科,广东省深圳市518036

出　　处：《中国组织工程研究》2018年第28期4501-4506,共6页Chinese Journal of Tissue Engineering Research

基　　金：深圳市卫生计生系统科研项目(201501024)~~

摘　　要：背景:股骨髋臼撞击症是近年逐渐被重视的引起年轻运动人群髋关节疼痛及功能障碍的一种疾病,现有研究尚无法阐明其发病机制及治疗中的诸多问题。目的:观察兔早期股骨髋臼撞击症模型形态学变化,并探讨其用于股骨髋臼撞击症研究的可行性。方法:12只新西兰兔,随机选取一侧行单侧股骨中上段内翻30°外旋45°截骨术,建立早期股骨髋臼撞击症模型,于术后4-16周分批行安乐死。拍摄髋关节X射线片进行对比后,取髋关节标本,行大体观察后,再行苏木精-伊红染色及甲苯胺蓝染色,对比观察股骨髋臼撞击区域软骨形态结构,并以Mankin评分评价软骨退变。结果与结论:(1)X射线片显示部分实验兔术侧股骨头颈交界区域软骨下骨出现类圆形骨质密度减低区;(2)大体标本观察则见术侧股骨髋臼撞击区域软骨损伤迹象;(3)组织学观察亦显示术侧股骨头颈交界区及髋臼盂唇软骨退变,Mankin评分显著高于对侧;(4)结果表明,新西兰兔早期股骨髋臼撞击症动物模型成功建立,依此可展开与股骨髋臼撞击症发病机制与治疗相关的研究工作。BACKGROUND： Femoroacetabular impingement（FAI） is a disease causing pain and dysfunction of hip joint in young active populations and has been paid more and more attention in recent years. However, its pathogenesisand optimized treatment have not been fully understood. OBJECTIVE： To establish an animal model of early FAI in New Zealand rabbits and investigate the feasibility of this model for the study on FAI by morphological observation. Methods： Twelve New Zealand rabbits were given unilateral femoral osteotomy with adduction 30° and external rotation 45° to establish the animal model of early FAI, and were then euthanized at 4 and 16 weeks in batches. Hip X-ray films were taken for comparison, the hip specimens were obtained for gross observation, then hematoxylin-eosin staining and toluidine blue staining were performed to observe the cartilage morphology in the area of impingement, and the cartilage degeneration was evaluated by Mankin scores. RESULTS AND CONCLUSION： Some low-density round areas were visible at the subchondral bone of the femoral head-neck conjunction in partial modeling femurs by X-ray. Gross specimen indicated the signs of cartilage injury at the impinging area. Histological observation also proved the cartilage degeneration at the femoral head-neck conjunction and acetabular labrum. The Mankin scores of the surgical side were significantly higher than those of the control side. Therefore, the animal model of early FAI in New Zealand rabbit is established successfully, which facilitates researches on the pathogenesis and treatment of FAI.

关 键 词：髋臼 截骨术 骨关节炎 模型 动物 组织工程 股骨髋臼撞击症 动物模型 兔 关节软骨 组织构建 

分 类 号：R318[医药卫生—生物医学工程]