深静脉血栓形成模型小鼠沉默信息调节因子1和氧化应激损伤标志物的表达  

Expression levels of SIRT1 and oxidative stress injury markers in a mouse model of deep venous thrombosis

作　　者：赵冲宇 王兵[1] 娄振凯[1] 李兴国[1] 何卫 赵学凌[1] Zhao Chong-yu;Wang Bing;Lou Zhen-kai;Li Xing-guo;He Wei;Zhao Xue-ling(First Affiliated Hospital of Kunming Medical University,Kunming 650032,Yunnan Province,China)

机构地区：[1]昆明医科大学第一附属医院,云南省昆明市650032

出　　处：《中国组织工程研究》2018年第28期4518-4524,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81160236;81760029;81760030);云南省卫生科技计划项目(2014NS142;2017NS022;2016NS021;2017NS021);昆明医科大学第一附属医院博士科研基金项目(2017BS030)~~

摘　　要：背景:研究表明,沉默信息调节因子1(silent information regulator 1,SIRT1)对氧化应激有抑制作用,降低动脉血栓形成。而SIRT1和氧化应激损伤在静脉血栓中的变化仍不清楚。目的:进一步分析SIRT1和氧化应激损伤与小鼠深静脉血栓形成的关系。方法:把90只的C57型小鼠按体质量随机分为3组:空白组(n=30),假手术组(n=30),深静脉血栓形成组(n=30)。深静脉血栓形成组用下腔静脉狭窄法制备深静脉血栓形成动物模型,24 h后获取小鼠下腔静脉组织。苏木精-伊红染色法观察血栓情况;DCFH-DA荧光探针捕获、流式细胞仪检测静脉组织中活性氧的含量。并测得超氧化物歧化酶和丙二醛的量;用Western blot法检测SIRT1蛋白的表达。结果与结论:(1)在深静脉血栓形成组里静脉壁中活性氧含量显著多于空白组和假手术组(P<0.05);(2)深静脉血栓形成组静脉壁组织超氧化物歧化酶的量显著低于空白组和假手术组,而丙二醛含量显著高于空白组和假手术组(P<0.05);(3)深静脉血栓形成组SIRT1表达显著低于空白组和假手术组(P<0.05);(4)结果提示:氧化应激损伤能促进深静脉血栓形成的发生发展,深静脉血栓形成中SIRT1的活性被抑制。BACKGROUND： Silent information regulator1（SIRT1） has been shown to inhibit oxidative stress and reduce arterial thrombosis. But changes in SIRT1 expression and oxidative stress in venous thrombosis remain unclear. OBJECTIVE： To further analyze the correlation of SIRT1 and oxidative stress in deep venous thrombosis. METHODS： Ninety C57 mice were randomized into three groups based on body mass： control, sham operation and model groups（n=30 per group）. The mouse model of deep venous thrombosis was created by inferior vena cava stenosis, and the inferior vena cava tissues were gained at 24 hours after modeling. The thrombosis was observed by hematoxylin-eosin staining; the content of reactive oxygen species was assayed by membrane permeable fluorescent probe DCFH-DA and flow cytometry; contents of superoxide dismutase and malondialdehyde were tested; SIRT1 protein expression was detected by western blot assay. RESULTS AND CONCLUSION： Compared with the control and sham operation groups, in the modeling group, the contents of reactive oxygen species and malondialdehyde were significantly increased, and the level of superoxide dismutase was significantly decreased（P〈0.05）. The expression level of SIRT1 in the modeling group was significantly lower than that in the control and modeling groups（P〈0.05）. In summary, oxidative stress plays a significant part in the germination and growth of deep venous thrombosis, and the activity of SITR1 is inhibited during deep venous thrombosis formation.

关 键 词：静脉血栓形成 模型 动物 氧化性应激 超氧化物歧化酶 丙二醛 组织工程 深静脉血栓 静脉内皮细胞 氧化应激损伤 沉默信息调节因子1 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]