电针对面神经损伤后面神经元中神经型钙黏素、上皮型钙黏素和胎盘型钙黏素表达的影响  

Effect of electroacupuncture on the expression of N-cadherin,E-cadherin and P-cadherin in the facial neurons after facial nerve injury

作　　者：龚立琼 蔡虹 费静 李雷激 Gong Li-qiong;Cai Hong;Fei Jing;Li Lei-ji(Department of Otorhinolaryngology Head and Neck Surgery,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属医院耳鼻咽喉头颈外科,四川省泸州市646000

出　　处：《中国组织工程研究》2018年第28期4525-4531,共7页Chinese Journal of Tissue Engineering Research

基　　金：泸州市科技局立项课题(2017LZXNYD-J34)~~

摘　　要：背景:作者所在课题组前期研究发现周围性面神经损伤能引起神经元中黏附分子的表达变化,而电针穴位治疗周围性面神经损伤的机制尚不清楚。目的:探讨电针穴位在面神经外周部受损后对中枢面神经元的形态学变化及面神经元中神经型钙黏素、上皮型钙黏素及胎盘型钙黏素表达的影响。方法:将新西兰大白兔随机分为正常对照组(n=4)、面神经损伤组(n=24)和电针组(n=24)。面神经损伤组与电针组均建立右侧面神经上颊支压榨损伤模型。造模成功后每天对电针组实验兔颊车、地仓、翳风、颧缪、四白、阳白电刺激,选择频率为18-20 Hz、电压为1.5 V的疏密波,另外埋针合谷穴不予以电刺激,针灸每次30 min,1次/d。结果与结论:(1)原位杂交结果显示各组兔面神经元中均未见上皮型钙黏素和胎盘型钙黏素mRNA表达,且正常对照组兔面神经元中未见神经型钙黏素mRNA表达,而面神经损伤组和电针组兔面神经元中出现神经型钙黏素mRNA的阳性表达,电针组兔面神经元中神经型钙黏素mRNA阳性细胞数均多于面神经损伤组(P<0.05);(2)免疫组化染色可见正常对照组兔面神经元中神经型钙黏素、上皮型钙黏素和胎盘型钙黏素蛋白表达;损伤后1 d时三者表达下降(电针组神经型钙黏素蛋白除外);损伤后1,4,14 d时电针组兔面神经元中神经型钙黏素和胎盘型钙黏素蛋白表达高于面神经损伤组(P<0.05);损伤后各时间点,电针组上皮型钙黏素蛋白表达均高于面神经损伤组(1 d时除外)(P<0.05);(3)苏木精-伊红染色可见面神经损伤组逐渐出现面神经元变性及类似神经元死亡表现,21 d时神经元开始恢复。电针组变性及类似死亡表现的神经元较面神经损伤组少,且14 d时神经元开始恢复,28 d时2组神经元形态均基本恢复;(4)提示面神经损伤引起了钙黏素的表达变化,电针治疗减轻了面神经元的变性并促进神经元恢复BACKGROUND： Preliminary studies have found that peripheral facial nerve injury can cause changes in the expression of adhesion molecules in neurons, but the mechanism of electroacupuncture in the treatment of facial nerve injury is unclear. OBJECTIVE： To observe the effect of electroacupuncture on the morphological changes of the facial neurons and the expression of N-cadherin, E-cadherin and P-cadherin in the neurons after peripheral facial nerve injury. METHODS： New Zealand white rabbits were randomized into control group（n=4）, model group（n=24） and electroacupuncture group（n=24）. Rabbits in the latter two groups were used to establish the crush injury model of superior buccal branch of the right facial nerve, and the electroacupuncture group rabbits were given the electroacupuncture at Jiache（ST6）, Dicang（ST4）, Yifeng（SJ17）, Quanliao（SI18）, Sibai（ST2） and Yangbai（GB14） with dilatational wave（18-20 Hz, 1.5 V）, and needle-embedding at Hegu（LI4） without electroacupuncture, 30 minutes, once daily. RESULTS AND CONCLUSION： In situ hybridization results suggested that E-cadherin mRNA and P-cadherin mRNA were negative in all groups, and N-cadherin mRNA was not expressed in the control group. The expression of N-cadherin mRNA was positive in the electroacupuncture and model groups after facial nerve injury. The number of N-cadherin mRNA positive cells in the electroacupuncture group was significantly higher than that in the model group（P〈0.05）. Immunohistochemical staining results found that the expressions of N-cadherin, E-cadherin and P-cadherin were positive in the control group; the expression levels of the three cadherins decreased at 1 day after injury（except N-cadherin in the electroacupuncture group）; the expression levels of N-cadherin and P-cadherin in the electroacupuncture group were higher than those in the model group at 1, 4 and 14 days after injury（P〈0.05）; the expression levels of E-cadherin in the electroacupuncture group was signific

关 键 词：面神经 电针 钙粘着糖蛋白类 原位杂交 免疫化学 组织工程 面神经损伤 面神经元 神经型钙黏素 上皮型钙黏素 胎盘型钙黏素 免疫组织化学染色 组织构建 

分 类 号：R318[医药卫生—生物医学工程]