NF-κB对病毒性心肌炎小鼠心肌中促炎细胞因子的调控  

Regulation of Proinflammatory Cytokine Expression by NF-κB in Viral Myocarditis

作　　者：薛陆静 潘海燕[2] Xue Luting;Pan Haiyan(Department of Cardiology,Xixi Hospital of Hangzhou,Zhejiang 310023,China)

机构地区：[1]杭州市西溪医院心内科,310023 [2]南通大学附属医院心内科,226001

出　　处：《医学研究杂志》2018年第8期45-47,53,共4页Journal of Medical Research

基　　金：国家自然科学基金青年科学基金资助项目(81100154)

摘　　要：目的探讨核因子-κB(NF-κB)对A型流感病毒(IAV)性心肌炎小鼠心肌中促炎细胞因子的调控作用。方法30只8周龄雄性BALB/c小鼠随机分3组:(1)正常对照组(C组):经鼻吸入15μl生理盐水;(2)感染对照组(I组):经鼻吸入40空斑形成单位(pfu)IAV;(3)PDTC组(P组):经鼻吸入40pfu IAV,腹腔注射NF-κB抑制剂吡咯烷二硫代氨基甲酸(PDTC)(10mg/kg),每天1次。感染后第9天处死小鼠,切取心脏组织进行病理及生化检查。结果 IAV感染可诱导心肌中促炎细胞因子白介素(IL)-1β、IL-6及肿瘤坏死因子(TNF)α显著上调(P<0.01,P<0.05),引发心肌急性炎性反应,部分心肌组织坏死。PDTC显著抑制促炎细胞因子在心肌中表达(P<0.01),有效抑制IAV病毒复制(P<0.01),减轻心肌炎性反应。结论 IAV感染心肌组织后可能通过NF-κB信号通路诱导心肌中促炎细胞因子表达上调,抑制NF-κB激活可能为预防和治疗流感病毒性心肌炎提供新的思路。Objective To investigate the regulatory effects of nuclear factor （NF）-κB on the expression of proinflammatory cytokines in influenza A virus （IAV）-induced myocarditis. Methods Thirty male BALB/c mice of 8 weeks old were randomly divided into 3 groups：1Normal control group （group C）：15μL saline with nasal inhalation;2Infection control group （group I）：infected intranasally with 40 plaque formation unit （pfu） IAV;3PDTC group （group P）：infected intranasally with 40 pfu IAV and injected intraperitoneally with NF-κB inhibitor pyrrolidine dithiocarbamic acid （PDTC）（10mg/kg）, once daily. The mice were euthanized at 9 days after infection and the heart tissue was taken for pathological and biochemical examination. Results IAV infection induced significant up-regulation of proinflammatory cytokines （IL） -1β,IL-6 and tumor necrosis factor（TNF） α in myocardium （P〈0.01, P〈0.05）, and triggered acute myocarditis, and some myocardial necrosis. PDTC significantly inhibited the expression of proinflammatory cytokines（P〈0.01）, and effectively suppressed the IAV replication（P〈0.01）, and myocardial inflammatory response. Conclusion IAV infection induces up-regulation of proinflammatory cytokines in myocardium might through the NF-κB signaling pathway inhibiting activation of NF-κB may provide new thinking for the prevention and treatment of influenza induced myocarditis.

关 键 词：A型流感病毒 病毒性心肌炎 核因子-κB促炎细胞因子 

分 类 号：R5[医药卫生—内科学]