胡杨基因组片段转化拟南芥表型研究  

Phenotypic Study of Arabidopsis thaliana Transformed by Genome Fragment from Populus euphratica

作　　者：郭飞龙 卢孟柱[2] 徐刚标[1] 叶天文 敖小平 GUO Fei-long;LU Meng-zhu;XU Gang-biao;YE Tian-wen;AO Xiao-ping(College of Forestry,Central South University of Forestry and Technology,Changsha 410004,Hu,nan,China;Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中南林业科技大学林学院,湖南长沙410004 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2018年第4期18-22,共5页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金“胡杨功能基因组研究平台构建和功能基因发掘”(CAFYBB2011001);中南林业科技大学大学生研究性学习和创新性实验项目

摘　　要：[目的]本研究旨在探索与挖掘胡杨基因组大片段的潜在功能,发掘具有潜在育种价值的胡杨基因簇。[方法]利用已构建的胡杨基因组BIBAC文库,采用花序浸染法,将胡杨基因组大片段78A2D10导入模式植物拟南芥基因组中。采用抗性筛选、分子检测及表型观察等方法鉴定、分析转化型植株。[结果]共获得15株特异表型的转化植株。与野生型相比,转化型植株主侧茎生长受到抑制,莲座叶面积增大近3倍,叶片数量增多,叶边缘皱缩,抽薹推迟约13周,株高增加近32.0 cm,侧茎发育成次生莲座,植株寿命延长约7周。[结论]胡杨基因组片段78A2D10可延长植株营养生长期及植株寿命,据此推测该基因片段可能与营养生长有关。[ Objective ] The study aims at exploring and excavating the potential function of large genome fragment cloned from Populus euphratica, and finding out the potential gene clusters with breeding value. [ Method ] Based on BIBAC library, the fragment 78A2DlO from the genome of P. euphratica, was inserted into the genome of Arabi- dopsis thaliana by the method of floral-dip. Resistance selection, molecular identification and phenotypic observa- tion were applied to identify and analyze the transgenic plants. [ Result] Fifteen transgenic plants with specific phe- notype were obtained in the research. Compared with the wild plants, the positive plants showed the characters such as inhibited growth of the stems, 3 times rosette leaf area, more leaves, crimping blade edge, delayed bolting with 13 weeks, height increasing by 32.0 cm, developing lateral stems and prolonged lifetime with 7 weeks. [ Conclu- sion] The fragment of 78A2DlO may prolong the vegetative growth and lifetime of plants. The fragment may be re- lated to the vegetative growth of plants.

关 键 词：胡杨 基因组大片段 花序浸染 拟南芥 表型 

分 类 号：S718.46[农业科学—林学]