裂叶垂枝桦组织培养与植株再生  

Tissue Culture and Plantlet Regeneration of Betula pendula Roth‘Dalecarlica'

作　　者：张丽杰[1] 和敬渊 孙昕淼 宋佳兴[1] 孙晓梅[1] 朱明[1] ZHANG Li-jie;HE Jtng-yuan;SUN Xin-miao;SONG Jia-xtng;SUN Xiao-mei;ZHU Ming(College Forestry,Shenyang Agricultural University,Shenyang 110866,Liaoning,China;College Forestry,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]沈阳农业大学林学院,辽宁沈阳110866 [2]北京林业大学林学院,北京100083

出　　处：《林业科学研究》2018年第4期135-141,共7页Forest Research

基　　金：国家科技基础条件平台建设项目(2005DKA21003)

摘　　要：目的]建立裂叶垂枝桦组织培养离体繁殖再生体系。[方法]以裂叶垂枝桦带腋芽或顶芽的茎段为试材,经过外植体消毒、初代培养、继代培养、增殖培养、生根培养,最后获得再生植株,并对裂叶垂枝桦组培快繁影响因素进行分析。[结果]表明:裂叶垂枝桦幼嫩茎段离体培养最适培养基和激素组合为:MS+0.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA+0.2 mg·L^(-1)GA_3+20 g·L^(-1)蔗糖+6 g·L^(-1)琼脂;最适生根培养基为:1/2MS+0.1 mg·L^(-1)NAA+20g·L^(-1)蔗糖+6 g·L^(-1)琼脂。将生根的无菌苗移植至草炭土和细沙比例3∶1的已灭菌的基质中,15 d后,组培苗生长健壮,成活率达到80%以上。[结论]采用组织培养技术对裂叶垂枝桦进行离体快繁,建立了离体快繁再生体系,为裂叶垂枝桦良种选育奠定了研究基础。[ Objective] To establish the tissue culture of vitro propagation regeneration system of Betula pendula Roth ‘Dalecarlica＇ [Method] Young stems of Betula pendula Roth. ‘ Dalecarlica＇ with axillary and apical bud were used as experimental materials to obtain regenerated plants by explants disinfection, original culture, succes- sive transfer culture, muhiplication culture and rooting culture. The factors influencing the rapid growth of the tissue culture were also analyzed. [ Result ] The results shows that： the young stem segments in vitro culture for optimum medium and hormone combination was MS ＋1.0 mg . L -16-BA ＋0.1 mg.L-1 NAA ＋0.2 mg. L-1 GAB ＋20 g. L-1 Sucrose ＋ 6 g.L-1 Agar. The optimum rooting medium was 1/2MS ＋0.1 mg . L-1 NAA ＋20 g. L-1 Sucrose ＋ 6 g .L- 1 Agar. The rooted seedlings were transplanted to the sterilized matrixes with peat soil and sand in the ra- tio of 3： 1. The tissue-cultured seedlings grew strongly and the survival rate was over 80% after 15 days. [ Conclu- sion ] Using tissue culture technique to rapid propagation in vitro for Betula pendula Roth ‘ Dalecarlica＇ , a rapid propagation in vitro ~generation system was established. It could lay a research foundation for Betula pendula Roth ‘Dalecarlica＇ elite breeding.

关 键 词：裂叶垂枝桦 组织培养 植株再生 

分 类 号：S723[农业科学—林木遗传育种]