HPLC法测定金芩芍注射液中绿原酸·黄芩苷和芍药苷的含量  

Determination of Chlorogenic Acid,Baicalin and Paeoniflorin in Jinqingshao Injection by High Performance Liquid Chromatography

作　　者：刘发全[1] 宋亚伟 吴昊[1] 张莉[1] 丁在亮[1] LIU Fa-quan;SONG Ya-wei;WU Hao(Anhui Province Veterinary Drug and Feed Information Net,Heiei,Anhui 230091)

机构地区：[1]安徽省兽药饲料监察所,安徽合肥230091

出　　处：《安徽农业科学》2018年第24期160-161,219,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立高效液相色谱法(HPLC)同时测定金芩芍注射液中绿原酸、黄芩苷和芍药苷的含量。[方法]色谱柱为Waters公司Symmetry C_(18)(4.6 mm×250 mm,5μm),流动相A为乙腈,B为0.025 mol/L磷酸溶液,采用梯度洗脱,流速为1.0 mL/min,测定波长为230 nm,柱温30℃,进样量10μL。[结果]绿原酸、芍药苷和黄芩苷分离度良好,分别在2.52~126.05、1.47~73.78、4.81~250.55μg/m L有良好的线性关系。[结论]该方法精密度高、重复性好、操作简便,适用于金芩芍注射液中绿原酸、芍药苷和黄芩苷的含量测定。[ Objective ] The research aimed to establish the high peflbmlance liquid chromatography method, and determine the contents of chlom- genie acid, baiealin and paeoniflorin of Jinqingshao injection at the same time. [ Method ] The samples were separated by waters company symmetry C18 column （4.6 minx250 mm, 5m） , using aeetonitrile and 0.025 mol/L phosphoric acid solution as mobile phases with flow rate 1.0 mL/min by gradient elution,detected by 230 nm, taked 10 ℃L at column temperatmre 30 ℃. I Result ] There was good separation of ehlomgenie acid, baieali- nand and peoniflorin.The linear ranges were 2.52-126.05,1.47-73.78 and 4.81-250.55℃g/mL,respeetively.In this ranges, the peak areas had a good eonrrelation. [ Conclusion] The method has high precision, high repeatability and simple operation, and is suitable tot the detemfination of ehlorogenie acid, baiealin and baiealin in Jinqingshao injection.

关 键 词：金芩芍注射液 绿原酸 黄芩苷 芍药苷 HPLC 

分 类 号：R284.1[医药卫生—中药学]