金丝桃苷抗抑郁作用参与5-HT能系统可能机制研究  

Studies on the Antidepressant-like Effect of Hyperoside on the Possible Mechanism of 5-HT System

作　　者：郑梅竹[1] 范亚军 潘炎 时东方[1] 刘春明[1] ZHENG Mei-zhu;FAN Ya-jun;PAN Yan;SHI Dong-fang;LIU Chun-ming(The Central Laboratory,Changchun Normal University,Changchun Jilin 130032,China;The College of Life Science,Changchun Normal University,Changchun Jilin 130032,China;Higher Education Research Institute,Changchun Normal University,Changchun Jilin 130032,China)

机构地区：[1]长春师范大学中心实验室,吉林长春130032 [2]长春师范大学生命科学学院,吉林长春130032 [3]长春师范大学高等教育研究所,吉林长春130032

出　　处：《长春师范大学学报》2018年第8期83-87,共5页Journal of Changchun Normal University

基　　金：国家自然科学基金资助项目"药用植物罗布麻抗抑郁成分的分离;活性评价及作用机制的研究"(30970299);国家自然科学基金资助项目"大气细颗粒物PM2.5对大鼠神经损伤机制的研究"(31400453);国家自然科学基金资助项目"人参属植物抗阿尔茨海默病的活性成分筛选方法及作用机制研究"(31370374);国家自然科学基金资助项目"药用植物抗帕金森病活性成分筛选及作用机制研究"(31170326);国家自然科学基金资助项目"药用植物中异黄酮类成分抗缺血性脑卒中的活性筛选及作用机制研究"(31670358);吉林省科技厅项目"白芍抗帕金森病的活性成分筛选及其作用机制研究"(20160101209JC)

摘　　要：本研究采用小鼠强迫游泳、小鼠悬尾和自主实验对金丝桃苷的抗抑郁作用进行了评价,同时选择5-HT_(1A)受体阻断剂Way100635、5-HT_2受体阻断剂cyproheptadine、5-HT_(2A)受体阻断剂ketanserin进行小鼠悬尾实验探讨金丝桃苷的抗抑郁机制。结果证实金丝桃苷具有确切的抗抑郁活性;5-HT_(2A)、5-HT_2受体阻断剂与金丝桃苷20mg/kg共同给药组与金丝桃苷20mg/kg单独给药组相比,共同给药组能明显延长小鼠悬尾的累计不动时间(P<0.05)。表明金丝桃苷的抗抑郁活性与5-羟色胺能系统(5-HT_(2A)、5-HT_2受体)有关。In the present study,the antidepressant activity of hyperoside was evaluated by using forced swimming,mouse suspension and autonomous experiments in mice.At the same time,5-HT1Areceptor blockers Way100635 and 5-HT2receptor blockers cyproheptadine and 5-HT2Awere selected to investigate the antidepressant mechanism of hyperoside by using mouse suspension test.The results confirmed that hyperoside has definite antidepressant activity;at the same time,5-HT2A,5-HT2receptor blockers ketanserin（5mg/kg）,cyproheptadine（3mg/kg）and hyperoside after 20mg/kg co-continuous administration,compared with hyperoside 20mg/kg alone,the cumulative immobility time of tail suspension was significantly prolonged（P〈0.05）.The hyper-depressant activity of hyperoside has been shown to be related to the serotoninergic system（5-HT2A,5-HT2receptor）.

关 键 词：金丝桃苷 抗抑郁 机制 

分 类 号：R285.5[医药卫生—中药学]