EB病毒再激活后鼻咽癌细胞系中基因表达差异分析  

Analysis of Gene Differential Expression in Nasopharyngeal Cancer After Epstein Barr Virus Reactivation

作　　者：李明阳[1] 马春霞[1] 吴翰欣 吴小海 俞建昆[1] LI Ming-Yang;MA Chun-Xia;WU Han-Xin;WU Xiao-Hai;YU Jian-Kun(Central Laboratory,Institute of Medical Biology,Chinese Academy of Medical Sciences ＆ Peking Union Medical College,Kunming 650118;Second Affiliated Hospital of Kunming Medical University,Kunming 650118 China)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所中心实验室,云南昆明650118 [2]昆明医科大学附属第二医院,云南昆明650118

出　　处：《生物技术通讯》2018年第4期471-476,共6页Letters in Biotechnology

基　　金：中国医学科学院重大协同创新项目(2016-12M-001)

摘　　要：目的:利用生物信息学方法从EB病毒再激活的鼻咽癌细胞系基因表达谱中筛选差异表达基因,并分析它们之间的互相作用关系。方法:利用edg R包等相关软件从基因表达谱中筛选差异表达的基因,分析它们之间的互相作用关系,并对其进行在线GO分析、KEGG富集分析及蛋白互作分析。结果:共筛选出4496个差异表达基因,包括1954个上调基因和2542个下调基因。GO分析结果显示,差异表达基因在生物过程(BP)中显著富集,包括病毒应答、Ⅰ型干扰素信号通路、γ干扰素介导的信号传导途径、病毒防御反应、胆固醇生物合成过程、氧化还原过程、类异戊二烯生物合成过程、类固醇代谢过程、脂质代谢过程和花生四烯酸代谢过程;在分子功能(MF)中,包括丝氨酸型内肽酶活性、蛋白同二聚化活性、醛脱氢酶(NAD)活性、氧气结合、3-氯烯丙基醛脱氢酶活性、血红素结合、谷胱甘肽转移酶活性、氧化还原酶活性、铁离子结合和药物结合;在细胞组分(CC)中,包括胞外体、细胞质、细胞器膜、基底外侧质膜、胞外空间、内质网膜、内质网、过氧化物酶体、线粒体、顶端质膜。KEGG通路分析显示,差异表达基因在代谢途径、抗生素生物合成、萜类骨架生物合成、化学致癌作用、丙型肝炎、胆汁分泌、细胞色素P450代谢外源性物质、氨基酸生物合成、类固醇生物合成、精氨酸和脯氨酸代谢等信号通路中显著富集。在蛋白互作分析网络中筛选出10个节点度最高的核心基因MAPK3、IRF7、IRF9、IFI6、TRIM22、IFI27、OAS2、TRIM31、HLA-F和MX1。结论:通过基因芯片对比筛选,EB病毒经化学物质再激活后引起宿主细胞多个基因表达发生变化,为相关临床研究提供了重要的信息基础。Objective： To screen differentially expressed genes（DEGs） from nasopharyngeal carcinoma tissue mi croarray and to analyze the protein interaction networks by bioinformatics. Methods： DEGs in nasopharyngeal carol noma tissues and normal tissues were screened by R language package, and then they were analyzed by GO analy sis, KEGG enrichment analysis and protein interaction analysis online. Results： In total, 4496 DEGs were identifled in nasopharyngeal cancer, including 1954 up-regulated genes and 2542 down-regulated genes. GO analysis resuits showed that DEGs were significantly enriched in biological processes（BP）, including response to virus, type I interferon signaling pathway, interferony mediated signaling pathway, defense response to virus, cholesterol biosyn thetic process, oxidation-reduction process, isoprenoid biosynthetic process, steroid metabolic process, lipid metabolic process, arachidonic acid metabolic process; in molecular function（MF）, including serine-type endopeptidase activity, protein homodimerization activity, aldehyde dehydrogenase（NAD） activity, oxygen binding, 3-chloroallyl aldehyde dehydrogenase activity, heme binding, glutathione transferase activity, oxidoreductase activity, iron ion binding, drug binding; in cell component（CC）, including extracellular exosome, cytosol, organelle membrane, basolateral plasma membrane, extracellular space, endoplasmic reticulum membrane, endoplasmic reticulum, peroxisome, mitochondrion, apical plasma membrane. KEGG pathway analysis showed the DEGs were enriched in metabolic path- ways, biosynthesis of antibiotics, terpenoid backbone biosynthesis, chemical carcinogenesis, hepatitis C, bile secre- tion, metabolism of xenobiotics by cytoclu＇ome P450, biosynthesis of amino acids, steroid biosynthesis, arginine and proline metabolism. In the protein interaction analysis network, the highest core genes of 10 nodes were selected MAPK3, IRF7, IRF9, IFI6, TRIM22, IFI27, OAS2, TRIM31, HLA-F and MX1. Conclusion： Gene chip comparison s

关 键 词：EB病毒 鼻咽癌 生物信息学 蛋白质相互作用 

分 类 号：Q811.4[生物学—生物工程] Q78