TBB诱导AGS细胞发生凋亡过程中对Prx V表达量的影响  

Effect of TBB Induced AGS Cell Apoptosis on Prx V Expression Quantity

作　　者：孙虎男[1] 焦冰洋 王闯[1] 刘悦[1] 宫伊希 韩英浩[1] 金成浩[1] Sun Hunan;Jiao Bingyang;Wang Chuang;Liu Yue;Gong Yixi;Han Yinghao;Jin Chenghao(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2018年第4期86-90,共5页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学科研培育基金(XA2014-04)

摘　　要：目前,胃癌在中国的发病率极高,且其致死率位于所有癌症首位,因此研发胃癌治疗药物和发现治疗药物的靶向因子显得极为迫切。TBB作为CK2的特异性抑制剂,被广泛应用到乳腺癌、甲状腺癌、宫颈癌等多种癌症的研究当中,并对癌细胞显示出极强的毒性。但TBB对胃癌的杀伤效果及其作用机制尚不明确。Prx V作为一种过氧化物还原酶,能够有效地清除细胞内ROS水平进而起到保护细胞,缓解细胞凋亡。研究通过对TBB诱导胃癌细胞凋亡过程中对细胞内ROS水平和Prx V蛋白质表达量的影响,探索Prx V在TBB诱导的胃癌细胞发生凋亡过程中的重要调控作用。结果显示,TBB可以不依赖ROS水平来诱导AGS胃癌细胞发生细胞凋亡,同时上调Prx V蛋白质的表达。At present, the morbidity of gastric cancer in China was extremely high, and its fatality rate was the in the first place of all the cancer , so it was urgent to research and develop gastric cancer treatment drugs and find that treatment with drugs targeting factor. TBB, as a specific inhibitor of CK2, widely used in breast cancer, thyroid cancer, cervical cancer and other cancer research, and cancer cells showed high toxicity. The effect of TBB killing gastric cancer and its mechanism was not clear. Prx V ,as a peroxide reductase,could effectively clear intracellular ROS level to protect the cell and ease apoptosis. Through researching the effect of TBB induced gastric cancer cells apoptosis on intracellular ROS level and Prx V protein expression quantity, important regulatory role of Prx V on TBB induced gastric cancer cells apoptosis was explored. Results showed that the TBB could be independent of ROS level to induce AGS gastric cancer cells apoptosis, at the same time increase Prx V protein expression.

关 键 词：Prx V TBB 胃癌细胞 细胞凋亡 

分 类 号：Q78[生物学—分子生物学]