NQO1基因在五味子乙素降低苯并(a)芘对HTR-8/SVneo细胞毒性中的作用研究  

NQO1 is Associated with Schisandrin B Attenuating Benzo(a)pyrene-induced HTR-8/SVneo Cells Damages

作　　者：史海霞[1] 刘传丽[1] 王华[1] 董渠龙 孙旸[1] 张岩 侯海燕[1,2] 陈亚琼[1] SHI Hai-xia;LIU Chuan-li;WANG Hua;DONG Qu-long;SUN Yang;ZHANG Yan;HOU Hai-yan;CHEN Ya-qiong(Department of Obstetrics and Gynecology,The Affiliated Hospital of the Chinese People＇s Armed Forces Logisticals College,Tianjin 300162,China;Chinese Academy of Medical Sciences ＆ Peking Union Medical College,Beijing 100730,Chin)

机构地区：[1]中国人民武装警察部队后勤学院附属医院妇产科,天津300162 [2]中国医学科学院北京协和医学院

出　　处：《国际妇产科学杂志》2018年第4期281-286,共6页Journal of International Obstetrics and Gynecology

基　　金：国家自然科学基金面上项目(81273977/H2902);国家自然科学基金青年项目(81402691/H2605);天津市应用基础与前沿技术研究计划青年项目(15JCQNJC12300)

摘　　要：目的:探索醌氧化还原酶1[NAD(P)H:quinine oxidoreductase1,NQO1]基因在五味子乙素(Schisandrin B,Sch B)降低苯并(a)芘[Benzo(a)pyrene,BaP]对人早孕胎盘绒毛膜外滋养层细胞(human trophoblast HTR-8/SVneo,HTR-8/SVneo)毒性中的作用。方法:在前期实验的基础上,以HTR-8/SVneo细胞为载体,利用基因转染技术、MTS细胞增殖实验、实时聚合酶链反应(real-time PCR)等方法从细胞层面分析NQO1基因干扰后Sch B细胞保护率的变化以及NQO1基因干扰前后各组NQO1 mRNA的表达量变化。结果:在细胞层面上,NQO1基因干扰后,20μmol/L的BaP对HTR-8/SVneo细胞增殖的抑制作用增强,抑制率达到30.48%;0.50、1.00、2.00μmol/L的Sch B仍然降低了BaP的毒性作用,但其细胞保护率均明显下降,细胞保护率分别为4.68%、9.56%、14.85%;同时,在基因表达层面上,NQO1基因干扰后Sch B激活NQO1的能力明显下降,其中0.10、0.25、0.50、1.00、2.00、5.00μmol/L的Sch B作用后NQO1 mRNA的表达量从NQO1基因干扰前是对照组的1.36、1.40、1.46、1.79、2.30、2.91、1.42倍,分别降至1.22、1.24、1.30、1.53、1.71、2.01、1.29倍。结论:NQO1基因的激活在Sch B降低BaP对HTR-8/SVneo细胞毒性作用中起到重要作用。Objective：To investigate whether NQO1 [NAD（P）H： quinine oxidoreductase 1] gene played a role in schisandrin B（Sch B） attenuating cells damages in the human trophoblast HTR-8/SVneo cells（HTR-8/SVneo） induced by Benzo（a） pyrene（BaP）. Methods：On the basis of previous experiments, siRNA technology, MTS cell proliferation assay and real-time PCR were used to analyze the changes of protective rate in HTR-8/SVneo cells induced by Sch B after NQO1 siRNA,and the changes of NQO1 mRNA expression in different groups before and after NQO1 siRNA. Results：At the level of cell proliferation, after NQO1 siRNA, inhibition rate in HTR-8/SVneo cells induced by 20 μmol/L BaP-incresed to 30.48%, while the protection rates in HTR-8/SVneo cells induced by 0.50, 1.00 and 2.00 μmol/L Sch B decresed to 4.68%, 9.56%, 14.85%respectively. Besides, at the level of gene expression, the ability of 0.10, 0.25, 0.50, 1.00, 2.00, 5.00 μmol/L Sch B to activate NQO1 significantly decreased after NQO1 si RNA, and the expression of NQO1 mRNA was respectively reduced to 1.22, 1.24,1.30, 1.53, 1.71, 2.01, 1.29 times as much as 1.36, 1.40, 1.46, 1.79, 2.30, 2.91, 1.42 times that of the control group before NQO1 siRNA. Conclusions：NQO1 was associated with Sch B attenuating BaP-induced HTR-8/SVneo cells damages.

关 键 词：苯并芘 五味子乙素 滋养细胞 醌氧化还原酶1 氧化还原酶类 

分 类 号：R285[医药卫生—中药学]