酶的定向进化及其应用进展  

Direct Evolution of Enzyme and Its Application Progress

作　　者：许勇[1] XU Yong(Anhui Academy of Medical Science,Hetei,Anhui 230061)

机构地区：[1]安徽省医学科学研究院,安徽合肥230061

出　　处：《安徽农业科学》2018年第23期12-14,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽省"十三五"医疗卫生重点专科"病原微生物实验室"(皖卫科教[2017]30号);2018年度安徽省卫生计生委科研计划项目"虫荧光素酶的定向进化研究"(2018YK006)

摘　　要：酶的定向进化是用于改良酶特性的一种策略,主要包括构建酶突变文库和高通量筛选。目前,突变文库的构建手段主要有随机突变、半理性设计和DNA改组;高通量筛选方法主要分为体内筛选和体外筛选。该文介绍易错PCR、半理性设计、DNA改组、表面展示、核糖体展示和差示荧光扫描等相关技术及其应用情况。Direct evolution of enzyme,a strategy to improve the characteristic of enzyme,mainly included constructing enzyme mutagenesis library and high-throughput screening. At present,the main methods of constructing enzyme mutagenesis library were random mutation,semi-rational design and DNA shuffling; high-throughput screening can be divided into in vivo screening and in vitro screening. In this study,the related techniques and their application situation,including error-prone PCR,semi-rational design,DNA shuffling,surface display,ribosome display and differential scanning fluorimetry,were introduced.

关 键 词：定向进化 易错PCR DNA改组 高通量筛选 

分 类 号：Q814[生物学—生物工程]