毛木耳958对4种抗生素的敏感性研究  

Study on the Sensitivity of Auricularia polytricha 958 to Four Kinds of Antibiotics

作　　者：杜春梅[1,2] 柏广波 董锡文[1,2] 薛春梅[1,2] DU Chun-mei;BAI Guang-bo;DONG Xi-wen(College of Life Science,Jiamusi University,Jiamusi,Heilongjiang 154007;Institute of Applied Microbiology,Jiamusi University,Jiamusi,Heilongjiang 154007)

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007 [2]佳木斯大学应用微生物研究所,黑龙江佳木斯154007

出　　处：《安徽农业科学》2018年第23期33-34,51,共3页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省自然科学基金面上项目(C2017064);黑龙江省教育厅基本科研业务费基础研究项目(2016-KYYWF-0581);佳木斯大学基础研究类(自然类)面上项目(JMSUJCMS2016-065);佳木斯大学校长创新创业基金项目(XZYF2018-12)

摘　　要：[目的]检测毛木耳958对青霉素、硫酸链霉素、制霉菌素和卡那霉素4种抗生素的敏感性,以寻找适合原生质体融合育种的抗性筛选标记。[方法]以毛木耳958为试验材料,在PDA培养基中加入不同浓度的抗生素,测定菌丝生长速率、菌落形态特征和锁状联合菌丝数量。[结果]适宜浓度的青霉素和硫酸链霉素对毛木耳958菌丝生长都有显著的促进作用,而制霉菌素和高浓度的卡那霉素对菌丝生长有明显的抑制作用;4种抗生素对毛木耳958菌落形态特征影响较小;青霉素、制霉菌素和卡那霉素高于300μg/m L时,毛木耳958锁状联合菌丝比率呈明显的下降趋势,硫酸链霉素对其无显著作用。[结论]毛木耳958对制霉菌素和高浓度的卡那霉素具有较强的敏感性,做筛选标记时控制好抗生素的浓度,尽量减少其对锁状联合菌丝比率的影响。[Objective] To study the sensitivity of Auricularia polytricha 958 to penicillin,streptomycin sulfate,nystatin and kanamycin,as well as to find the resistance screening markers suitable for protoplast fusion breeding.[Method]Taking Auricularia polytricha 958 as the material,growth rate of mycelia,morphological features of colony and clamp connection amount of mycelia were determined by adding different concentrations of four kinds of antibiotics in PDA media.[Result] Growth rate of mycelia were promoted significantly by a proper concentration of ampicillin and streptomycin sulfate.But growth rate of mycelia were inhibited significantly by nystatin and the higher concentration of kanamycin.The effects of four antibiotics on the colonial morphological characteristics of the stain were not significantly.The ratios of hyphal clamp connection of Auricularia polytricha 958 were decreased when the concentration of penicillin,nystatin and kanamycin were higher than 300 μg/m L.The effect of streptomycin sulphate on forming of clamp connection was not significant.[Conclusion] The sensitivities of Auricularia polytricha 958 to nystatin and the higher concentration of kanamycin were obvious.Thus,the concentration of antibiotics should be controlled when it was used to screening marker,and minimize the effect of the antibiotic on ratio of hyphal clamp connection.

关 键 词：毛木耳958 抗生素 敏感性 菌丝 锁状联合 

分 类 号：S646.6[农业科学—蔬菜学]