霍山石斛多糖的半仿生提取工艺优化与抗炎活性评价  

Optimization on Semi-bionic Extraction Technology of Polysaccharides from Dendrobium huoshanense and Evaluation on Its Anti-inflammatory Activity

作　　者：戴玮 罗建平 DAI Wei;LUO Jian-ping(School of Food Science and Engineering,Hefei University of Technology,Hefei,Anhui 23000)

机构地区：[1]合肥工业大学食品科学与工程学院,安徽合肥230009

出　　处：《安徽农业科学》2018年第23期151-154,183,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化霍山石斛多糖的半仿生提取工艺,并评价所提多糖的抗炎活性。[方法]以多糖得率为指标,采用单因素试验和正交试验方法对提取工艺进行优化,以LPS诱导的RAW264.7细胞为体外炎症模型,评价多糖的抗炎活性。[结果]半仿生提取法最佳工艺条件为:料液比1∶30、提取温度80℃、模拟胃液提取时间1.5 h、模拟肠液提取时间2.5 h,在此条件下多糖的得率为(60.9±1.2)mg/g,较传统水提法的多糖得率提高16.55%。细胞试验表明,霍山石斛多糖能抑制RAW264.7细胞NO及IL-1β的分泌,降低i NOS和IL-1βm RNA的表达。[结论]优化后的提取工艺合理可行,提取率高,所提多糖的抗炎活性优于传统方法制备的多糖。[Objective]The semi-bionic extraction technology of Dendrobium huoshanense polysaccharides was optimized and the anti-inflammatory activity of polysaccharides was evaluated.[Method]Single factor and orthogonal experiments were used to optimize the extraction technology based on polysaccharide yield. LPS-induced RAW264. 7 macrophages were used to determine the anti-inflammatory activity of polysaccharides.[Result]The optimal conditions of semi-bionic extraction were solid-liquid ratio of 1 ∶30,extraction temperature of 80 ℃,stimulated gastric fluid extraction time of 1.5 h and simulated intestinal fluid extraction time of 2.5 h. Under the optimized conditions,the yield of polysaccharides was（60.9±1.2） mg/g,which was increased by 16.55% as compared to the traditional water extraction technology.Cell experiments showed that the polysaccharides obtained by semi-bionic extraction technology could effectively inhibit the secretion of NO and IL-1β from RAW264.7 cells and reduce the m RNA expression of i NOS and IL-1β in RAW264.7 cells.[Conclusion]The optimized technology is reasonable and feasible,its extraction ratio is high,and the extracted polysaccharides from Dendrobium huoshanense has better anti-inflammatory activity than that of the polysaccharides prepared by water extraction.

关 键 词：霍山石斛多糖 半仿生提取法 抗炎活性 

分 类 号：S-3[农业科学]