紫花白芨组织培养技术研究  

Study on tissue culture system of purple flower Bletilla striata

作　　者：杨硕知[1,2] 谭著明 沈宝明[1,2] 申爱荣 谭云 YANG Shuozhi;TAN Zhuming;SHEN Baoming;SHEN Airong;TAN Yun(Hunan Academy of Forestry,Changsha 410004,China;Hunan Engineering Technology Research Center for Culturing and Utilizing of Understorey Bioresources,Changsha 410004,China)

机构地区：[1]湖南省林业科学院,湖南长沙410004 [2]湖南省林下特色生物资源培育与利用工程技术研究中心,湖南长沙410004

出　　处：《湖南林业科技》2018年第3期10-14,共5页Hunan Forestry Science & Technology

基　　金：湖南省林业厅林业发展改革处项目

摘　　要：为建立白芨种子高效实用的组织培养技术体系,以紫花大种白芨成熟未开裂蒴果为外植体,进行组培快繁技术研究。结果表明:外植体表面清洁后,在5%次氯酸钠溶液中浸泡15 min,之后将种子接种于萌发培养基,配方为MS+6-BA(1.0 mg·L^(-1))。种子萌发并形成绿色原球茎即可转入幼苗营养生长培养基,配方为MS+6-BA(2.0 mg·L^(-1))+NAA(0.5 mg·L^(-1))。幼苗生长到3 cm以上时,接入生根培养基中,配方为1/2MS+6-BA(0.1 mg·L^(-1))+NAA(0.5 mg·L^(-1))。待其根系长度达到1 cm后,可进行炼苗移栽。The seed tissue culture system has been preliminarily established by using the mature but not cracking capsule of purple flower Bletilla striata as explant. The basic procedure is as follows. Explants are soaked in 5% HCl O solution for 15 min after surface cleaning,and inoculated on the germinate culture medium until green protocorns formed,the fomula of which is MS ＋ 6-BA（ 1. 0 mg·L^-1）. The protocorns are subcultured to vegetative growth culture medium until seedling growth over 3 cm,the fomula of which is MS ＋ 6-BA（ 2. 0 mg·L^-1） ＋ NAA（ 0. 5 mg·L^-1）. Then seedlings would be transferred to root medium,the fomula is 1/2 MS ＋ 6-BA（ 0. 1 mg·L^-1） ＋NAA（ 0. 5 mg·L^-1）. Acclimatization and transplant would be performed after the root growth over 1 cm.

关 键 词：白芨 组织培养 培养基 

分 类 号：S567.239[农业科学—中草药栽培]