玉米细胞核雄性不育突变体K305ms的生理生化分析  

Analysis of physiological and biochemical characteristics of maize male sterile mutant K305ms

作　　者：汪燕[1,2] 石海春[1,3] 余学杰[1,3] 赵长云 柯永培[1,3] WANG Yan;SHI Haichun;YU Xuejie;ZHAO Changyun;KE Yongpei(College of Agronomy,Sichuau Agricultural University,Chengdu 611130,China;Research Center of Buck wheat Industry Technology,Institute of Plant Genetics and Breeding,Guizhou Normal University,Guiyang 550001,China;Sichuan Nongda Zhenghong Bio.Co.,Ltd,Chengdu 610213,China)

机构地区：[1]四川农业大学农学院,四川成都611130 [2]贵州师范大学植物遗传育种研究所荞麦产业技术研究中心,贵州贵阳550001 [3]四川农大正红生物技术有限责任公司,四川成都610213

出　　处：《浙江农业学报》2018年第8期1281-1287,共7页Acta Agriculturae Zhejiangensis

基　　金：四川科技厅省重点支撑项目(Grand 2011FZ0119;YZGG2011-3-11)

摘　　要：为探讨60Co-γ辐射诱变获得的玉米细胞核雄性不育突变体K305ms的生理生化特征,以不育株K305ms及其姊妹交群体中的可育株K305F为材料,对雄花发育不同时期花药和颖壳中的可溶性蛋白含量、游离脯氨酸含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性进行了测定。结果表明,与可育株K305F相比,不育株K305ms雄花发育过程中花药与颖壳的可溶性蛋白含量和游离脯氨酸含量均不同程度降低,且K305ms花药的SOD和CAT活性明显降低,而POD活性显著或极显著升高。说明玉米细胞核雄性不育可能与花药发育过程中物质代谢和抗氧化系统异常有关。In order to understand physiological and biochemical characteristics of the maize genic male sterile mutant K305 ms induced by60 Co-γ irradiation,male sterile mutant K305 ms and fertile plant K305 F from their sister-crossed line were used as materials. The content change of soluble protein,free proline,and the activity of superoxide dismutase（SOD）,catalase（CAT）,peroxidase（POD） during the anther development stage were detected. The main results were showed as follows： The soluble protein content and free proline content in anther and glume of K305 ms mutant were lower than fertile plant K305 F during anther development stage; Moreover,the activities of SOD and CAT in anther of K305 ms mutant were significant lower than those in K305 F,whereas the POD activity was significant higher. The results indicated that genic male sterility of maize was related to abnormal metabolism of soluble protein and the disordered reactive oxygen scavenging enzyme system（ROS） during the development of anther.

关 键 词：玉米 细胞核雄性不育 突变体K305ms 生理生化特性 

分 类 号：S513[农业科学—作物学] Q343.34[生物学—遗传学]