七味清肠胶囊对慢性功能性便秘模型大鼠症状以及血清和结肠组织中VIP、SP、MOT、CGRP含量的影响  

Effects of Qiwei Qingchang Capsules on Symptoms and the Contents of VIP,SP,MOT and CGRP in Serum and Colon Tissue of Rats with Chronic Functional Constipation

作　　者：张华 李溥[2] 贺银菊[3] 黄文强[2] 唐毅 杨再波[3] ZHANG Hua;LI Pu;HE Yinju;HUANG Wenqiang;TANG Yi;YANG Zaibo(Dept.of Gastroenterology,Qiannan Buyi and Miao Autonomous Prefecture Hospital of TCM,Guizhou Duyun 558000,China;Dept.ofMedical Laboratory Science,Qiannan Medical College for Nationalities,Guizhou Duyun 558000,China;Qiannan Normal University for Nationalities/Guizhou Province College Research Centre of Ethnical MedicinalPlant Resources Exploitation Engineering,Guizhou Duyun 558000,China)

机构地区：[1]黔南布依族苗族自治州中医医院消化内科,贵州都匀558000 [2]黔南民族医学高等专科学校医学检验系,贵州都匀558000 [3]黔南民族师范学院/贵州省高校民族药用植物资源开发工程研究中心,贵州都匀558000

出　　处：《中国药房》2018年第16期2170-2174,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.21466031);贵州省科技合作计划项目(No.黔科合LH字[2015]7716);贵州省中医药管理局中医药;民族医药科学技术研究课题(No.黔中医药发[2016]10号-QZYY2016095)

摘　　要：目的:探讨七味清肠胶囊对慢性功能性便秘(CFC)模型大鼠症状以及血清和结肠组织中血管活性肠肽(VIP)、P物质(SP)、胃动素(MOT)、降钙素基因相关肽(CGRP)含量的影响。方法:将60只SD大鼠随机分为正常对照组、模型组、阳性对照组(琥珀酸普芦卡必利片,0.02 g/kg,以普芦卡必利计)以及七味清肠胶囊低、中、高剂量组(0.8、1.6、3.2 g/kg,以生药总量计),每组10只。除正常对照组外,其余各组大鼠均灌胃复方地芬诺酯片(10 mg/kg)复制CFC模型。成模后,各给药组大鼠均灌胃相应药物,正常对照组和模型组大鼠灌胃等容生理盐水,每天1次,连续7 d。观察各组大鼠体征、体毛颜色、粪便形质等一般症状以及首粒黑便排出时间、小肠推进率,采用酶联免疫吸附测定法检测大鼠血清和结肠组织中VIP、SP、MOT、CGRP的含量。结果:与正常对照组比较,模型组和各给药组大鼠更易激惹,且精神萎靡,皮毛萎黄无光泽,排便次数及粒数减少,粪便粗糙;首粒黑便排出时间显著延长,小肠推进率以及血清和结肠组织中SP、MOT的含量均显著降低,VIP、CGRP的含量均显著升高(P<0.05)。与模型组比较,各给药组上述症状均有所改善;首粒黑便排出时间显著缩短,小肠推进率以及血清和结肠组织中SP、MOT的含量均显著升高,VIP、CGRP的含量均显著降低(P<0.05),且七味清肠胶囊低、中剂量组上述指标均显著差于阳性对照组(P<0.05),而高剂量组与阳性对照组相当(P>0.05)。结论:七味清肠胶囊可缓解CFC模型大鼠的便秘症状,缩短其首粒黑便排出时间,提高小肠推进率,作用机制可能与上调血清和结肠组织中SP、MOT含量,下调VIP、CGRP含量有关。OBJECTIVE：To investigate the effects of Qiwei qingchang capsules on symptoms and the contents of VIP,SP,MOT and CGRP in serum and colon tissue of rats with chronic functional constipation（CFC）. METHODS：Totally 60 SD rats were randomly divided into normal control group,model group,positive control group（Prucalopride succinate tablets 0.02 g/kg,by prucalopride）,Qiwei qingchang capsules low-dose,medium-dose and high-dose groups（0.8,1.6,3.2 g/kg,by crude drug）,with 10 rats in each group. Except for normal control group,other groups were given intragastric administration of Compound diphenoxylate tablets（10 mg/kg） to induce CFC model. After modeling,administration groups were given relevant medicine intragastrically,normal control group and model group were given constant volume of normal saline intragastrically,once a day,for consecutive 7 d. General symptoms as sign,body hair color and fecal shape,first black stool discharge time and small intestinal propulsion ratio were observed in each group. ELISA method was used to detect the contents of VIP,SP,MOT and CGRP in serum and colon tissue of rats. RESULTS：Compared with normal control group, the rats were more irritable, mentally depressed,skinless and glossy,less stool frequency and fewer grains, and coarse stool in model group and administration group. The first black stool discharge time was prolonged significantly, and small intestinal propulsion ratio, the contents of SP and MOT in serum and colon tissue were decreased significantly,while the contents of VIP and CGRP were increased significantly（P〈0.05）. Compared with model group,above symptoms of administration groups were all improved;first black stool discharge time was shortened significantly,and small intestinal propulsion ratio,the contents of SP and MOT in serum and colon tissue were increased significantly,while the contents of VIP and CGRP were decreased significantly（P〈0.05）;above indexes of Qiwei qingchang capsules low-dose and medium-dose groups were significantly wor

关 键 词：七味清肠胶囊 慢性功能性便秘 大鼠 症状 血清 结肠组织 血管活性肠肽 P物质 胃动素 降钙素基因相关肽 

分 类 号：R975.3[医药卫生—药品] R285[医药卫生—药学]