响应面法优化云南宣木瓜齐墩果酸和熊果酸的提取工艺研究  

Optimization of Extraction Technology of Oleanolic Acid and Ursolic Acid from Yunnan Chaenomeles speciosa by Response Surface Methodology

作　　者：罗小芳 郭乙颖[1] 胡柳 王钗 宋艳芝 李治章 王宗成 LUO Xiao-fang;GUO Yi-ying;HU Liu;WANG Chai;SONG Yan-zhi;LI Zhi-zhang;WANG Zong-cheng(College of Chemical and Biological Engineering,Hunan University of Science and Engineering,Yongzhou 425199,Hunan,China;Hunan Provincial Engineering Research Center for Ginkgo biloba,Hunan University of Science and Engineering,Yongzhou 425199,Hunan,China;Hunan Key Laboratory of Comprehensive Utilization of Advantage Plants Resources of Southern Hunan,Hunan University of Science and Engineering,Yongzhou 425199,Hunan,China)

机构地区：[1]湖南科技学院化学与生物工程学院,湖南永州425199 [2]湖南科技学院湖南省银杏工程技术研究中心,湖南永州425199 [3]湖南科技学院湘南优势植物资源综合利用湖南省重点实验室,湖南永州425199

出　　处：《食品研究与开发》2018年第17期31-36,共6页Food Research and Development

基　　金：湘南优势植物资源综合利用湖南省重点实验室开放基金(XNZW15C04);湖南省高校科技创新团队支持计划(2012-318);湖南科技学院生物工程重点学科

摘　　要：探索超声辅助提取宣木瓜齐墩果酸和熊果酸的工艺优化研究。在单因素试验的基础上,选取对提取工艺影响较大的乙醇浓度、超声时间和超声功率3个因素,利用Box-Benhnken中心组合原理进行三因素三水平试验,并通过响应面分析法优化超声波辅助提取宣木瓜中齐墩果酸和熊果酸的工艺参数。结果表明最优工艺条件为:乙醇浓度95%,超声时间3.8 min、超声功率440 W,齐墩果酸和熊果酸的总得率为4.03 mg/g,与理论基本相符。因此,超声辅助提取法能快速有效地提取宣木瓜中的齐墩果酸和熊果酸。The extraction technology of oleanolic acid and ursolic acid from Chaenomeles speciosa by ultrasonic-assisted extraction was studied. Ethanol concentration, ultrasonic time and ultrasonic power were selected as factors based on single-factor test and a three-factors-three-levels experiment design had been developed by Box-Benhnken central composite design method. The extraction technology conditions of oleanolic acid and ursolic acid from Chaenomeles speciosa by ultrasonic-assisted extraction was optimized by response surface analysis experiments. The results showed the optimal conditions were that： concentration of ethanol 95 %, time 3.8 min, ultrasonic power 440 W. Under the optimum conditions, the actual total yield of oleanolic acid and ursolic acid was 4.03 mg/g, which matched with the predicted yield. Oleanolic acid and ursolic acid from Chaenomeles speciosa could be extracted quickly and efficiently by ultrasonic-assisted extraction.

关 键 词：宣木瓜 齐墩果酸 熊果酸 响应面 提取工艺 

分 类 号：R284.2[医药卫生—中药学]