草莓新品种京藏香茎尖组培快繁技术研究  

Study on Stem-tip Tissue Culture and Rapid Propagation Techniques of New Strawberry Cultivar Jingzangxiang

作　　者：杨波[1] 赵宝龙 孙军利 刘莲玲 YANG Bo;ZHAO Bao-long;SUN Jun-li;LIU Lian-ling(College of Agronomy,Shihezi University,Shihezi 832000,China;The Corps Key Laboratory of the Cultivation Physiology and Germplasm Resources Utilization of Characteristic Fruits and Vegetables,Shihezi 832000,China)

机构地区：[1]石河子大学农学院,新疆石河子832000 [2]特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832000

出　　处：《黑龙江农业科学》2018年第7期30-35,共6页Heilongjiang Agricultural Sciences

基　　金：兵团技术转移专项资助项目(2014BD032);兵团科技特派员创新创业计划资助项目(2015CA011)

摘　　要：为加快草莓无病毒苗的推广应用,以我国自育草莓品种京藏香为试材,进行茎尖诱导萌发、继代增殖和生根培养不同激素种类和浓度的筛选研究,并采用RT-PCR检测其带病毒情况,对其脱毒效果进行评价。结果表明:适宜京藏香茎尖诱导萌发培养基为MS+0.6mg·L^(-1) 6-BA+0.1mg·L^(-1) IAA,继代增殖培养基为MS+1.5mg·L^(-1) 6-BA+0.1mg·L^(-1) NAA,生根培养基为1/2 MS+0.1mg·L^(-1) IBA;经病毒检测组培苗的轻型黄边病毒(SMYEV)和斑驳病毒(SMoV)的脱毒率为100%;镶脉病毒(SVBV)的脱毒率为82%,说明脱毒效果良好。In order to accelerate the application of strawberry virus-free seedling,this experiment chose Chinese self-bred strawberry variety Jingzangxiang as material,we analyzed the selection of different types and concentrations of different hormones in stem tip induced germination,secondary appreciation and rooting culture,and detected the virus condition by RT-PCR and evaluated its detoxification effect.The results showed that the suitable medium for Jingzangxiang shoot tip induction was MS＋0.6 mg·L^-1 6-BA＋0.1 mg·L^-1 IAA,the subculture medium was MS＋1.2 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA,and the rooting medium was 1/2 MS＋0.1 mg·L^-1 IBA.The detoxication rate of the SMYEV and the SMoV was all 100%,and the detoxication rate of the SVBV was 82%,indicated that the detoxification effect was good.

关 键 词：草莓 京藏香 组培 茎尖 

分 类 号：S668.4[农业科学—果树学]