提高化生平合剂二次开发的质量标准研究  

Study on the improvement of quality standard in secondary development of Huashengping Mixture

作　　者：赵文娜[1] 白静[1] 周凯[1] 车晓侠[1] 张爱军[1] 王清晖[1] 张抗怀[1] ZHAO Wenna;BAI Jing;ZHOU Kai;CHE Xiaoxia;ZHANG Aijun;WANG Qinghui;ZHANG Kanghuai(The Second Affiliated Hospital of Xi′an Jiaotong University,Xi＇an 710004,China)

机构地区：[1]西安交通大学第二附属医院,西安710004

出　　处：《西北药学杂志》2018年第4期444-447,共4页Northwest Pharmaceutical Journal

基　　金：西安交通大学第二附属医院科研基金青年项目[编号:YJ(QN)201619]

摘　　要：目的建立化生平合剂中大黄素和丹参酮ⅡA的含量测定方法,提高化生平合剂的质量标准。方法采用TLC法对处方中的虎杖和丹参进行鉴别,采用HPLC法同时测定虎杖中大黄素和丹参中丹参酮ⅡA的含量。色谱条件为:采用Krosamil C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水(75∶25)为流动相;流速:1.0mL·min-1;柱温:25℃。结果大黄素、丹参酮ⅡA分别在0.156 4~1.564和0.021 6~0.216μg范围内峰面积与其进样量线性关系良好,加样回收率分别为98.42%和103.29%,RSD值分别为1.11%和1.54%。结论该方法简便、准确,可用于化生平合剂的质量控制。Objective The method for determination of emodin and tanshinone ⅡA was established in order to improve the quality standard of Huashengping Mixture.Methods TLC was used to identify Polygonum cuspidatum and Salvia miltiorrhiza.HPLC with a Krosamil C18（250 mm×4.6 mm,5μm）column was applied for the determination of emodin and tanshinone ⅡA using methanol-water（75∶25）as the mobile phase.The flow rate was 1.0 mL·min^（-1） and the temperature of column was 25 ℃.Results The linear ranges of emodin and tanshinoneⅡA were 0.156 4-1.564 and 0.021 6-0.216μg,respectively.The average recoveries were 98.42% and 103.29% and the RSD were 1.11% and 1.54%,respectively.Conclusion This method is simple and accurate,and can be used for the quality control of Huashengping Mixture.

关 键 词：化生平合剂 大黄素 丹参酮ⅡA 质量标准 HPLC法 TLC法 

分 类 号：R282[医药卫生—中药学]