UPLC-MS/MS法测定对乙酰氨基酚栓的含量  

Determination of the content of Paracetamol Suppositories by UPLC-MS/MS

作　　者：蒋志涛[1] 吕玲燕 王雪[1] 陈晓峰[1] 余辉 邢国良 JIANG Zhitao;LU Lingyan;WANG Xue;CHEN Xiaofeng;YU Hui;XING Guoliang(Zhangjiagang Hospital of Traditional Chi nese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Graduate Workstation of Enterprises in Jiangsu Province,Zhangjiagang 215600,China)

机构地区：[1]南京中医药大学附属张家港医院江苏省企业研究生工作站,张家港215600

出　　处：《西北药学杂志》2018年第4期465-468,共4页Northwest Pharmaceutical Journal

基　　金：苏州市科技发展计划项目(编号:SYSD2016161;SYSD2017161)

摘　　要：目的采用UPLC-MS/MS法测定对乙酰氨基酚栓中对乙酰氨基酚的含量。方法色谱柱:ACQUITYTM UPLC BEH C18(2.1mm×50mm,1.7μm);流动相:甲醇-5mmol·L^(-1)乙酸铵(含2mL·L^(-1)乙酸)(65∶35);流速:0.2mL·min-1;进样量:2μL;柱温:30℃,质谱采用ESI+多反应监测模式(MRM)进行扫描。结果对乙酰氨基酚在3min内分离良好,在相应的质量浓度范围内与峰面积呈良好的线性关系(r=0.995 7,n=6);加样回收率为99.2%(n=6),RSD值为2.0%。结论建立的UPLCMS/MS分析方法简便、快速、灵敏度高、专属性强、结果准确,适用于对乙酰氨基酚栓的质量控制。Objective To determine the content of acetaminophen in Paracetamol Suppositories by UPLC-MS/MS.Methods The LC conditions were as follows：the column,ACQUITYTM UPLC BEH C_（18）（2.1 mm×50 mm,1.7μm）;the mobile phase,methanol-5 mmol·L^（-1） ammonium acetate（containing 2 mL·L^（-1） acetic acid）（65∶35）;the flow rate was 0.2 mL·min^（-1）;the injection volume was 2μL;the column temperature was 30 ℃.The mass spectra were recorded by using ESI＋multiple reaction monitoring（MRM）.Results Acetaminophen was well separated within 3 min and had a good linear relationship with the peak area in the corresponding concentration range（r=0.995 7,n=6）.The recovery was 99.2%（n=6）,and RSD was 2.0%.Conclusion The established UPLC-MS/MS method is simple,rapid,sensitive,specific and accurate.It is suitable for the quality control of Paracetamol Suppositories.

关 键 词：对乙酰氨基酚 UPLC-MS/MS 含量测定 

分 类 号：R927.2[医药卫生—药学]