义乌小鲵微卫星位点的跨种扩增  

Cross-species Amplification of Microsatellite Loci for Hynobius yiwuensis

作　　者：刘盼 陈雅琴 邵晨[1] 王宇[1] LIU Pan;CHEN Ya-qin;SHAO Chen(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《安徽农业科学》2018年第20期83-85,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省自然科学基金项目(LQ14C040001)

摘　　要：[目的]筛选具有多态性的义乌小鲵(Hynobius yiwuensis)微卫星位点。[方法]采用跨种扩增的方法从安吉小鲵(H.amjiensis)和中国小鲵(H.chinensis)的113个微卫星位点中筛选具有多态性的义乌小鲵微卫星位点。[结果]筛选出11个具有多态性的义乌小鲵微卫星位点。这些多态性微卫星位点的等位基因数为2~6个(平均3.55),观察杂合度为0.200~1.000(平均0.510),期望杂合度为0.500~0.732(平均0.662),多态信息含量为0.375~0.737(平均0.588)。[结论]筛选得到的具有多态性的11个义乌小鲵微卫星位点可应用于其种群遗传学和保护遗传学的研究。[Objective]To screen the polymorphic microsatellite loci of Hynobius yiwuensis.[Method]Polymorphic microsatellite loci of H.yiwuensis were developed from 113 microsatellite loci from H.amjiensis and H.chinensis using cross-species amplification.[Result]11 polymorphic microsatellite loci of H.yiwuensis were developed.The number of alleles,observed and expected heterozygosities and polymorphic information content of these polymorphic loci were ranged from 2 to 6 with an average of 3.55 alleles per locus,0.200 to 1.000（ average 0.510）,0.500 to 0.732（ average 0.662） and 0.375 to 0.737（ average 0.588）,respectively.[Conclusion]These 11 polymorphic microsatellite loci of H.yiwuensis can be used in population genetic and conservation genetic researches.

关 键 词：义乌小鲵 微卫星 跨种扩增 多态性 

分 类 号：Q959.53[生物学—动物学]