防治奶牛乳房炎中药组方的筛选及其抑菌杀菌作用研究  

Study on Screening of Traditional Chinese Medicine Formula and Its Anti-bacterial Effects Preventing Bovine Mastitis

作　　者：孙静[1] 冶冬阳[2] 王旭荣[1] 侯艳华[1] 王磊[1] 张景艳[1] 张凯[1] 张康[1] 李建喜[1] 杨志强[1] SUN Jing;YE Dongyang;WANG Xurong;HOU Yanhua;WANG Lei;ZHANG Jingyan;ZHANG Kai;ZHANG Kang;LI Jianxi;YANG Zhiqiang(l.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050,Chin;Institute of Agricultural Sciences in Coastal Area of Jiangsu Prowince,Yancheng 224002,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [2]江苏沿海地区农业科学研究所,盐城224002

出　　处：《中国畜牧兽医》2018年第7期1990-2000,共11页China Animal Husbandry & Veterinary Medicine

基　　金：国家奶牛产业技术体系(CARS36);中兽医与临床创新团队(CAAS-ASTIP-2015-LIHPS-O6)

摘　　要：试验根据中兽医治疗奶牛乳房炎的辨证施治原则,筛选出抑制引起奶牛乳房炎主要病原菌(金黄色葡萄球菌和大肠杆菌)的最佳中药组方,为其临床防治提供新的途径与方法。选用蒲公英、马齿苋、金银花、紫花地丁、赤芍、白芍、菊花、黄芩、漏芦、红花10味中药,通过L_(12)(2^(11))正交试验初步筛选抗菌中药组方中的最佳优选因素,再通过L_(16)(4~5)正交试验进一步筛选药物组方配比;采用牛津杯法测定中药组方对奶牛乳房炎主要致病菌的体外抑菌效果;采用二倍稀释法和平板计数法分别测定中药组方对奶牛乳房炎主要致病菌最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果显示,通过L_(12)(2^(11))正交试验初步筛选出了抗菌中药组方最佳优选因素:赤芍、黄芩、红花、菊花和白芍;L_(16)(4~5)正交试验进一步筛选药物组方最佳配比为黄芩∶赤芍∶菊花∶白芍∶红花=3∶3∶3∶1∶2;新药组方对大肠杆菌的抑菌直径为17.39mm,MIC和MBC分别为62.50、125.00mg/mL,对金黄色葡萄球菌的抑菌直径为25.44mm,MIC和MBC均为31.25mg/mL。本研究成功筛选出了一种对奶牛乳房炎主要致病菌具有良好抑菌、杀菌作用的中药新配比组方。In order to provide new methods and approaches to the treatments of bovine mastitis,this study screened out the best prescription for inhibiting the main pathogens causing mastitis in dairy cows according to the principles of dialectical methods that Chinese veterinarians treat diseases.The Dandelion,Purslane,Honeysuckle,Purple Flower,Radix Paeoniae Rubra,Radix Paeoniae Alba,Chrysanthemi Flos,Scutellariae Radix,Reed and Carthami Flos were used as materials in this experiment.A preliminary screening of antibacterial Chinese herbal medicines was conducted through L（12）（2^（11））orthogonal test,the drug group was further screened by the orthogonal test of L（16）（4~5）,and the anti-bacterial effect of Chinese medicine group on the main pathogenic bacteria of bovine mastitis was determined by Oxford cup method in vitro.The minimum antimicrobial concentration（MIC）and minimum bactericidal concentration（MBC）were detected by two-fold dilution method and plate counting.The results showed that the optimal factors of group were Paeoniae Radix Rubra,Scutellariae Radix,Carthami Flos,Paeoniae Radix Alba and Chrysanthemi Flos,and the proportion was 3∶3∶3∶1∶2.The bacterial inhibition diameters for Escherichia coli was 17.39 mm,and MIC and MBC were 62.50 and 125.00 mg/mL respectively.The bacterial inhibition diameters for Staphylococcus aureus was 25.44 mm,and MIC and MBC both were 31.25 mg/mL.In conclusion,the study successfully screened out a new traditional Chinese medicine that had good antibacterial and antiseptic effects for the bacteria causing bovine mastitis.

关 键 词：奶牛乳房炎 中药组方 正交试验 最小抑菌浓度(MIC) 最小杀菌浓度(MBC) 牛津杯法 

分 类 号：S859.3[农业科学—临床兽医学]