肿节风三清颗粒抗炎和抗哮喘作用  

Anti-inflammatory and Antiasthmatic Activities of Zhongjiefeng Sanqing Granules

作　　者：梁正敏[1] 李锦辉[1] 崔玉梅 彭健波 陶卿 何家康[1,4] LIANG Zhengmin;LI Jinhui;CUI Yumei;PENG Jianbo;TAO Qing;HE Jiakang(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;College of Veterinary Medicine,Jilin University,Changchun 130062,China;Guangxi BDX Animal Drugs Co.,Ltd.,Nanning 530003,China;Guangxi Engineering Research Center for Veterinary Medicine Animal Science,Nanning 530003,China)

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]吉林大学动物医学学院,长春130062 [3]广西北斗星动物保健品有限公司,南宁530003 [4]广西兽药制剂工程技术研究中心,南宁530003

出　　处：《中国畜牧兽医》2018年第7期2008-2014,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31360624);国家科技型中小企业技术创新基金(13C26214504804)

摘　　要：本研究旨在考察肿节风三清颗粒的抗炎和平喘作用。MTT法检测200、400、800、1 600μg/mL肿节风三清颗粒对RAW264.7细胞活力的影响;用脂多糖(LPS)诱导RAW264.7细胞建立体外炎症模型,并采用400、800、1 600μg/mL肿节风三清颗粒处理,ELISA法检测细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)水平;用卵清蛋白(OVA)致敏、激发雌性BALB/c小鼠建立哮喘模型,并采用100、200、300mg/kg肿节风三清颗粒处理,末次激发24h后,测定哮喘小鼠气道高反应性(AHR),取支气管肺泡灌洗液(BALF)进行白细胞分类计数,ELISA法检测BALF中IL-4、IL-5和IL-13浓度及血清中OVA-IgE水平。结果显示,200~16 00μg/mL肿节风三清颗粒对RAW 264.7细胞增殖有显著或极显著促进作用(P<0.05;P<0.01),与LPS组相比,800、1 600μg/mL肿节风三清颗粒能够极显著降低TNF-α和IL-6水平(P<0.01);与哮喘模型组相比,肿节风三清颗粒明显改善了气道高反应性,明显减少了BALF中的炎症细胞数目及IL-4、IL-5和IL-13浓度,极显著降低了血清中OVA-IgE水平(P<0.01)。综上所述,肿节风三清颗粒可通过抑制炎症因子的表达对炎症细胞模型和哮喘小鼠模型起到良好的保护作用。The study was aimed to investigate the anti-inflammatory and antiasthmatic effects of Zhongjiefeng Sanqing granules.The cell viability that treated with 200,400,800 and 1 600μg/mL Zhongjiefeng Sanqing granules was measured by MTT method.The inflammation model of RAW264.7 cell was established with LPS in vitro,and then TNF-αand IL-6 levels in the cell supernatant were measured by ELISA after 400,800 and 1 600μg/mL Zhongjiefeng Sanqing granules treatment.The asthma model established by sensitizing and challenging female BALB/c mice with ovalbumin（OVA）to evaluate the effects of Zhongjiefeng Sanqing granules on asthmatic mice in vivo,the airway hyperresponsiveness（AHR）was measured at 24 hafter the final OVA challenge,and the bronchoalveolar lavage fluid（BALF）were harvested to measure the number of inflammatory cells and the concentrations of IL-4,IL-5 and IL-13,and the serum was collected to measure the level of OVA-IgE.The results showed that Zhongjiefeng Sanqing granules in the con-centration range of 200 to 1 600μg/mL could significantly or extremely significantly promote the proliferation of RAW 264.7 cell（P0.05;P0.01）.Compared with the LPS group,800 and1 600μg/mL Zhongjiefeng Sanqing granules could extremely significantly reduce the levels of TNF-αand IL-6（P0.01）.Compared with the asthma model group,Zhongjiefeng Sanqing granules obviously ameliorated the airway hyperresponsiveness,markedly reduced the number of inflammatory cells in BALF,concentrations of IL-4,IL-5 and IL-13 in BALF and level of OVA-IgE in serum.These results suggested that Zhongjiefeng Sanqing granules had protective effect on inflammatory cell model and asthmatic model of mouse by inhibiting the expression of inflammatory cytokines.

关 键 词：肿节风三清颗粒 抗炎 平喘 炎症因子 

分 类 号：R931.6[医药卫生—生药学]