超声辅助提取尾叶香茶菜总二萜工艺研究  

Extraction Process of Total Diterpenoids from Rabdosia Excise by Ultrasonic-Assisted Extraction Method

作　　者：周天娇 李春燕[1,2] 韩莉萍 李亚娟 吴艳芳 ZHOU Tianjiao;LI Chunyan;HAN Liping;LI Yajuan;WU Yanfang(School of Pharmacy,Xinxiang Medical University,Xinxiang 453002,China;Pharmaceutical Comprehensive Laboratory,Sanquan College of Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院药学院,河南新乡453002 [2]新乡医学院三全学院药学综合实验室,河南新乡453003

出　　处：《中医药学报》2018年第3期77-80,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81703458);国家级大学生创新创业训练计划项目(201310472002;201310472014);河南省科技攻关计划(172102310326);河南省高等学校重点科研项目(17A360026)

摘　　要：目的:研究超声辅助提取尾叶香茶菜中总二萜的工艺。方法:以Kamebakaurin作为对照品,采用紫外分光光度法来测定尾叶香茶菜中总二萜的含量,通过单因素和响应面实验考察超声提取尾叶香茶菜总二萜的主要影响因素,来确定超声辅助提取最佳的工艺条件。结果:超声辅助提取最佳条件为:功率400 W,提取温度60℃,乙醇浓度64%。结论:超声提取尾叶香茶菜总二萜的测定的方法简单可行,效果好,提取效率高。Objective： To study the extraction process of the total diterpenoids from Rabdosia excise by method of ultrasonic-assisted extraction. Methods： The content of the total diterpenoids was determined by UV-spectrophotometry using Kamebakaurin as the standard. The single factor experiment and response surface methodology were used to optimize the technology of the ultrasonic-assisted extraction method. Results： The optimum extraction conditions were as follows：the extracting power was 400 W,the extracting temperature was 60℃,the extracting solvent was 64% ethanol. Conclusion： The extraction of the total diterpenoids from Rabdosia excise by ultrasonic-assisted extraction was simple,feasible,and effective.

关 键 词：超声提取 尾叶香茶菜 响应面法 

分 类 号：R284.2[医药卫生—中药学]