一测多评法测定山楂叶提取物中4种成分的含量  

SIMULTANEOUS DETERMINATION OF FOUR COMPONENTS IN HAWTHORN LEAF EXTRACT BY QUANTITATIVE ANALYSIS OF MULTI-COMPONENTS BY SINGLE MARKER

作　　者：徐宝欣 杜义龙[1] 潘海峰[1] 赵胜男[1] XU Bao-xin;DU Yi-long;PAN Hai-feng(Institute of Chinese Materia Medica/Hebei Key Laboratory of Research and Exploitation of Traditional Chinese Medicine,Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院中药研究所/河北省中药研究与开发重点实验室,河北承德067000

出　　处：《承德医学院学报》2018年第4期273-276,共4页Journal of Chengde Medical University

基　　金：河北省高等学校科学技术研究重点项目(ZD2015097);河北省高等学校科学技术研究项目(QN2017005);河北省高校重点学科建设项目资助

摘　　要：目的:建立同时测定山楂叶提取物中牡荆素鼠李糖苷、牡荆素葡萄糖苷、金丝桃苷、牡荆素含量的一测多评法。方法:以牡荆素葡萄糖苷为内标物,计算牡荆素鼠李糖苷、金丝桃苷、牡荆素3个成分的相对校正因子,并将该方法的测量结果与传统外标曲线法的测定结果进行比较。结果:牡荆素葡萄糖苷与牡荆素鼠李糖苷、金丝桃苷、牡荆素的相对校正因子分别为0.963、1.116、1.034;一测多评法与传统方法测定结果比较,牡荆素鼠李糖苷、金丝桃苷、牡荆素的含量无明显差异。结论:经方法学验证,本研究建立的一测多评法可用于山楂叶提取物中4种成分含量的同时测定。Objective： To establish the method for simultaneous determination of rhamnosylvitexin, glucosylvitexin, hyperoside and vitexin content in Hawthorn leaf extract by quantitative analysis of multi-components by single marker. Methods： The relative correction factor of rhamnosylvitexin, hyperoside and vitexin were calculated by using glucosylvitexin as internal standard, and the content determination results were compared with the traditional external standard curve method. Results： The relative correction factor between glucosylvitexin and rhamnosylvitexin, hyperoside, vitexin were respectively 0.963, 1.116 and 1.034. There was no obvious difference about the rhamnosylvitexin, hyperoside and vitexin content between quantitative analysis of multi-components by single marker and traditional external standard curve method. Conclusions： The quantitative analysis of multi-components by single marker in this study can be used in simultaneous determination of 4 components in Hawthorn leaf extract.

关 键 词：山楂叶提取物 一测多评法 牡荆素鼠李糖苷 牡荆素葡萄糖苷 金丝桃苷 牡荆素 

分 类 号：R284.1[医药卫生—中药学]