锶对脂肪干细胞成骨分化中血管内皮生长因子及Notch1信号通路相关蛋白表达的影响  

Effects of Strontium on the Expression of VEGF and Notch1 Signaling Pathway in Osteogenic Differentiation of Adipose-derived Stem Cells

作　　者：赵崇如 郭澍[1] 吕梦竹[1] 陈欣[1] 王婷[1] 朱梦茹 ZHAO Chongru;GUO Shu;LU Mengzhu;CHEN Xin;WANG Ting;ZHU Mengru(Department of Plastic Surgery,The First Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院整形外科,沈阳110001

出　　处：《中国医科大学学报》2018年第8期696-700,共5页Journal of China Medical University

基　　金：国家自然科学基金(51272286);国家卫生和计划生育委员会公益性行业科研专项(2015SQ00049);辽宁省自然科学基金(20102296)

摘　　要：目的探究锶(Sr)上调脂肪干细胞(ADSCs)成骨分化过程中血管内皮生长因子(VEGF)的表达与Notch1信号通路的关系。方法分别用氯化锶和γ-分泌酶抑制剂(DAPT)干预成骨分化诱导的ADSCs,运用Western blotting方法检测Notch1信号通路蛋白(NICD)及VEGF的蛋白表达。结果与空白对照组相比,Sr组的NICD蛋白表达水平显著升高,DAPT组的NICD蛋白表达水平显著降低;与Sr组相比,Sr+DAPT组的NICD蛋白表达显著降低。与空白对照组相比,Sr组的VEGF蛋白表达水平显著升高,DAPT组的VEGF蛋白表达水平显著降低;与Sr组相比,Sr+DAPT组的VEGF蛋白表达显著降低。结论 Sr能上调ADSCs成骨分化过程中VEGF表达。在Sr促ADSCs成骨分化过程中,Notch1信号通路被激活,并对VEGF的表达起正向调控作用。Objective To investigate the relationship between Notch1 signaling pathway and vascular endothelial growth factor（VEGF） in strontium（Sr）-induced adipose-derived stem cell（ADSC） osteogenic differentiation. Methods The osteogenically induced ADSCs were treated with strontium and γ-secretase inhibitors（DAPT）. Western blotting method was used to detect the protein expression level of Notch1 signaling pathway related protein（NICD） and VEGF. Results The expression level of NICD was significantly higher in the Sr-treated group and lower in the DAPT-treated group than in the control group. The expression level of NICD was significantly lower in the Sr and DAPT-treated group than in the Sr-treated group. The expression level of VEGF was significantly higher in the Sr-treated group and lower in the DAPT-treated group than in the control group. The expression level of VEGF was significantly lower in the Sr and DAPT-treated group than in the Sr-treated group. Conclusion Strontium can upregulate the expression level of VEGF in osteogenically induced ADSCs. Notch1 signaling pathway was activated during the strontium-induced osteogenic differentiation of ADSCs,and plays a positive role in regulating VEGF expression.

关 键 词：脂肪干细胞 锶 NOTCH信号通路 血管内皮生长因子 成骨 

分 类 号：R62[医药卫生—整形外科]