太空搭载海水钝顶螺旋藻诱变株H_(11)的室外产多糖特性研究  

Study on polysaccharide production outdoor by seawater Spirulina platensis mutant H_(11) induced through space-flight carriage

作　　者：谭丽[1,2] 李涛 吴华莲[1] 吴嘉仪[1] 向文洲[1] 刘德海[3] TAN Li;LI Tao;WU Hua-lian;WU Jia-yi;XIANG Wen-zhou;LIU De-hai(Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;University of Chinese Academy of Sciences,Beijing 100049,China;Guangzhou Baiyun Lianjia FineChemical Factory,Guangzhou 510412,China)

机构地区：[1]中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室,广东广州510301 [2]中国科学院大学,北京100049 [3]广州市白云联佳精细化工厂,广东广州510412

出　　处：《海洋通报》2018年第3期328-334,共7页Marine Science Bulletin

基　　金：广州市科技计划项目(201604016056);国家海洋经济创新示范城市项目(2017BH0005);广东丹姿集团科技研发项目(DZ201501)

摘　　要：本研究对一株经太空搭载后选育的海水钝顶螺旋藻诱变株H_(11)的室外产多糖能力进行了评估。结果显示,在广州秋季室外平均温度为27°C下,诱变株H_(11)的生物量产率、总糖含量、总多糖含量和水溶性多糖含量分别为19.09±0.03 mg/(L·d)、27.89±1.28%DW、20.95±0.87%DW和19.74±0.93%DW。与野生株WT相比,H_(11)的生物量、总糖、总多糖和水溶性多糖的产率分别提高了15.39%,148.93%,176.50%和187.70%。在冬季室外平均培养温度为17°C条件下,诱变株和野生株生长速率及多糖产率都有所降低,但诱变株在产多糖能力上仍显著高于野生株(P<0.05)。研究表明海水钝顶螺旋藻诱变株H_(11)产多糖能力极高,具有可观的产业应用前景。The main objective of this paper is to evaluate the capacity of polysaccharide production outdoor by seawater Spirulina platensis mutant H_（11） which was screened after space-flight carriage. The results of outdoor cultivation showed that,under average temperature 27 °C during the autumn at Guangzhou, the biomass productivity, total saccharide content,total polysaccharide content and water-soluble polysaccharide content of the mutant strain H_（11） was 19.09 ± 0.03 mg/（L·d）,27.89 ± 1.28 %, 20.95 ± 0.87 % and 19.74 ± 0.93 % respectively, and compared with wild type, the productivity of biomass,total saccharide, total polysaccharide and water-soluble polysaccharide of the mutant strain H_（11） increased by 15.39 %, 148.93 %,176.50 % and 187.70 % respectively. When the average temperature declined to 17 ℃ in the winter, compared with the results in the autumn, both the growth rate and the yield of polysaccharide of mutant H_（11） and wild type were decreased obviously, but the mutant H_（11） still displayed absolute advantage over the wild type in polysaccharide production. It was indicated that the mutants H_（11） has great potential of producing polysaccharide and promising prospects for industrial application.

关 键 词：海水钝顶螺旋藻 多糖 太空搭载 室外培养 

分 类 号：P745[天文地球—海洋生物学] S917.3[天文地球—海洋科学]