糖尿病肾病患者SOD-Mn基因多态性与肾功能、氧化性损伤的相关性  

The correlation of SOD-Mn gene polymorphism with renal function and oxidative injury in patients with diabetic nephropathy

作　　者：张伟开 张玲[2] ZHANG Wei-kai;ZHANG Ling(Nephrology and Endocrinology Department,Yingshan County People＇s Hospital in Hubei Province,Huanggang,Hubei Province,438700,China;Nephrology Department,Huanggang Central Hospital in Hubei Province,Huanggang,Hubei Province,438000,China)

机构地区：[1]湖北省英山县人民医院肾脏病内分泌科,湖北黄冈438700 [2]湖北省黄冈市中心医院肾脏病学科,湖北黄冈438000

出　　处：《海南医学院学报》2018年第14期1311-1314,1318,共5页Journal of Hainan Medical University

基　　金：长江大学黄冈临床医学院(WJ2016-YZ-10)~~

摘　　要：目的:研究糖尿病肾病患者SOD-Mn基因多态性与肾功能、氧化性损伤的相关性。方法:选择2014年3月~2017年3月在英山县人民医院诊断为糖尿病肾病患者作为研究的DN组,另取同期体检的健康志愿者作为研究的对照组。测定外周血中SOD-Mn基因rs4880位点的多态性及氧化应激分子的表达量、血清中肾功能指标及氧化应激指标的含量。结果:DN组患者SOD-Mn基因CC基因型的构成比低于对照组,CT+TT基因型的构成比高于对照组;DN组中CC基因型及CT+TT基因型患者的血清T-AOC含量以及外周血Nrf2、SIRT1表达强度均显著低于对照组,血清MDA、AOPP、8-OHdG、8-Iso-PGF2α含量以及外周血NOX4、NOX5、p38MAPK、NF-κB表达强度均显著高于对照组(P<0.05),且CT+TT基因型患者的血清T-AOC含量以及外周血Nrf2、SIRT1表达强度均显著低于CC基因型患者,血清MDA、AOPP、8-OHdG、8-Iso-PGF2α含量以及外周血NOX4、NOX5、p38MAPK、NF-κB表达强度均显著高于CC基因型患者(P<0.05)。结论:糖尿病肾病患者SOD-Mn基因rs4880位点C向T的突变会加重肾功能损伤及氧化应激反应。Objective：To study the correlation of SOD-Mn gene polymorphism with renal function and oxidative injury in patients with diabetic nephropathy.Methods：Patients who were diagnosed with diabetic nephropathy in Yingshan County People＇s Hospital between March 2014 and March 2017 were chosen as DN group,and healthy volunteers who received physical examination during the same period were selected as the control group.The SOD-Mn gene rs4880 locus polymorphism and oxidative stress molecule expression in peripheral blood as well as the contents of renal function indices and oxidative stress indices in serum were determined.Results：The constituent ratio of SOD-Mn gene CC genotype in DN group was lower than that in control group whereas the constituent ratio of CT＋TT genotype was higher than that in control group;serum T-AOC contents as well as peripheral blood Nrf2 and SIRT1 expression intensity of DN group of patients with CC genotype and CT＋TT genotype were significantly lower than those of control group whereas serum MDA,AOPP,8-OHdG and 8-Iso-PGF2α contents as well as peripheral blood NOX4,NOX5,p38 MAPK and NF-κB expression intensity were significantly higher than those of control group（P〈0.05）.Moreover,serum T-AOC content as well as peripheral blood Nrf2 and SIRT1 expression intensity of patients with CT＋TT genotype was significantly lower than those of patients with CC genotype whereas serum MDA,AOPP,8-OHdG and 8-Iso-PGF2α contents as well as peripheral blood NOX4,NOX5,p38 MAPK and NF-κB expression intensity were significantly higher than those of patients with CC genotype（P〈0.05）.Conclusion：The mutation from SOD-Mn gene rs4880 locus C to T can aggravate the renal function injury and oxidative stress response in patients with diabetic nephropathy.

关 键 词：糖尿病肾病 SOD-Mn 基因多态性 氧化应激反应 

分 类 号：R587.2[医药卫生—内分泌]