子痫前期孕妇尿液中sFlt-1/PLGF比值与滋养细胞侵袭、凋亡异常的相关性  

The correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia

作　　者：洪喜萍[1] 奚杰[1] HONG Xi-ping;XI Jie(Obstetrics Department,Shanghai Jiading Maternity ＆Infant Health Hospital,Shanghai 201821,China)

机构地区：[1]上海市嘉定区妇幼保健院产科,上海201821

出　　处：《海南医学院学报》2018年第14期1351-1354,共4页Journal of Hainan Medical University

基　　金：上海市科技局基金项目(2015-KY-10)~~

摘　　要：目的:探讨子痫前期孕妇尿液中可溶性fms样酪氨酸激酶1(sFlt-1)/胎盘生长因子(PLGF)比值与滋养细胞侵袭、凋亡异常的相关性。方法:2015年12月~2017年12月间在本院进行产检的孕中期健康孕妇183例作为正常对照组,同期在本院进行产检的孕中期子痫前期孕妇62例作为子痫前期组。对比两组孕妇尿液中sFlt-1/PLGF比值的差异,检测其胎盘组织中侵袭相关基因、凋亡相关基因的表达量。进一步采用Pearson检验评估子痫前期孕妇尿液sFlt-1/PLGF比值与滋养细胞侵袭、凋亡异常的相关关系。结果:子痫前期组孕妇的尿液sFlt-1/PLGF比值显著高于正常对照组孕妇;子痫前期组孕妇胎盘组织中Rho、ROCK、Notch-1、MMP9、LAMA4 mRNA的表达量低于正常对照组孕妇,CDKN1C mRNA的表达量高于正常对照组孕妇;子痫前期组孕妇胎盘组织中Bcl-2、Survivin mRNA的表达量低于正常对照组孕妇,Bax、Caspase-3、Caspase-8mRNA的表达量高于正常对照组孕妇;差异均有统计学意义(P<0.05)。相关性分析发现,子痫前期孕妇尿液sFlt-1/PLGF比值与子痫前期孕妇胎盘滋养细胞侵袭相关基因、凋亡相关基因表达量直接相关。结论:子痫前期孕妇尿液中sFlt-1/PLGF比值异常增高,可进一步加剧胎盘滋养细胞的侵袭及凋亡异常。Objective：To investigate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Methods：183 healthy second-trimester pregnant women who received antenatal care in this hospital between December 2015 and December 2017 were selected as the normal control group,and 62 secondtrimester pregnant women with preeclampsia who received antenatal care in this hospital during the same period were selected as the preeclampsia group.The difference of sFlt-1/PLGF ratio in the urine was compared between the two groups of pregnant women,and the expression levels of invasion-related genes and apoptosis-related genes in the placental tissues were detected.The Pearson test was adopted to further evaluate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Results：Urine sFlt-1/PLGF ratio of preeclampsia group was significantly higher than that in normal control group;Rho,ROCK,Notch-1,MMP9 and LAMA4 mRNA expression in placental tissues of preeclampsia group were lower than those of normal control group whereas CDKN1 CmRNA expression was higher than that of normal control group;Bcl-2 and Survivin mRNA expression in placental tissues of preeclampsia group were lower than those of normal control group whereas Bax,Caspase-3 and Caspase-8 mRNA expression were higher than those of normal control group（P〈0.05）.Correlation analysis showed that the urine sFlt-1/PLGF ratio of pregnant women with preeclampsia was directly correlated with the expression of placental trophoblast invasion-related genes and apoptosis-related genes in pregnant women with preeclampsia.Conclusion：The sFlt-1/PLGF ratio abnormally increases in the urine of pregnant women with preeclampsia,which can further aggravate the abnormal invasion and apoptosis of placental trophoblast cells.

关 键 词：子痫前期 sFlt-1/PLGF比值 侵袭 凋亡 

分 类 号：R714.2[医药卫生—妇产科学]