实验红鲫C1HD系SNP标记的研究  

Establishment of SNPs markers of the laboratory red crucian carp C1HD strain

作　　者：朱丰 吴端生[2] ZHU Feng;WU Duansheng(Endocrinology Division,the Affiliated Hospital of Jinggangshan University,Jiangxi Province,Ji＇an 343009,China;Department of Laboratory Animal Science,University of South China,Hu＇nan Province,Hengyang 421001,China)

机构地区：[1]井冈山大学附属医院内分泌科,江西吉安343009 [2]南华大学实验动物学部.湖南衡阳421001

出　　处：《中国医药导报》2018年第19期4-7,共4页China Medical Herald

基　　金：“十二五”国家科技支撑计划课题(2015BAI09B0503);湖南省教育厅科学研究项目(12A122)

摘　　要：目的建立实验红鲫C1HD系的SNP标记。方法参照斑马鱼的SNPs基因库,设计SNP引物,采用单管双向等位基因专一性扩增(SB-ASA)方法,对12尾实验红鲫C1HD系和12尾实验红鲫封闭群的血液DNA样品进行SNPs基因分型并测序验证。结果 zK261O7基因的zSNP30124211位点(G/A型)、zC227H20基因的zSNP24678043位点(A/T型)和zC39F23基因的zSNP26474328位点(G/C型)等3个SNPs位点能够准确辨别实验红鲫C1HD系和实验红鲫封闭群。结论 zSNP30124211位点(G/A型)、zSNP24678043位点(A/T型)和zSNP26474328位点(G/C型)均可作为实验红鲫C1HD系的SNPs标记。Objective To establish SNP markers of the laboratory red crucian carp C1HD strain. Methods SNPs primers were designed referring to SNPs gene library of the Zebrafish, the SNPs were genotyped by single-tube bi-directional allele-specific amplification（SB-ASA） and were verified by sequencing for the laboratory red crucian carp C1HD strain. Results SNPs-PCR of blood DNA samples of laboratory red crucian carp could be genotyping of three sites Zk261O7 gene zSNP30124211（G/A type）, zC227H20 gene zSNP24678043（A/T type） and zC39F23 gene zSNP26474328（G/C type）. These three SNP markers were able to accurately identify the laboratory red crucian carp C1HD strain and the laboratory red crucian carp closed colony. Conclusion Three SNPs markers of Zk261O7 gene loci zSNP30124211（G/A type）, zC227H20 gene loci zSNP24678043（A/T type） and zC39F23 gene loci zSNP26474328（G/C type） could be used as SNPs markers in the laboratory red crucian carp.

关 键 词：实验红鲫 C1HD系 单核苷酸多态性 单管双向等位基因专一性扩增 

分 类 号：Q95-33[生物学—动物学]