外源H_2S和H_2O_2对NaCl胁迫下加工番茄幼苗生理特性的影响  

Effect of Exogenous H_2S and H_2O_2 on the Physiological Characteristics of Processing Tomato Seedlings under NaCl Stress

作　　者：蔺亚平 林海荣[1] 崔辉梅[1,2] LIN Yaping;LIN Hairong;CUI Huimei(Agricultural College,Shihezi University,Shihezi 832003,China;Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi 832003,China)

机构地区：[1]石河子大学农学院,新疆石河子832003 [2]特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832003

出　　处：《华北农学报》2018年第3期159-166,共8页Acta Agriculturae Boreali-Sinica

基　　金：留学回国人员科技活动项目(2010LX005)

摘　　要：为探明外源H_2S和H_2O_2对NaCl胁迫下加工番茄幼苗的生理调节作用,以耐盐性弱的KT-32和耐盐性强的KT-7这2个加工番茄品系为材料,采用盆栽法,设置0.5,1.5,3.0 mmol/L 3个H_2O_2浓度水平,研究50μmol/L H_2S和H_2O_2共处理对250 mmol/L NaCl胁迫下加工番茄幼苗的生长、渗透调节、物质积累和活性氧代谢的影响。结果表明,外源H_2S和H_2O_2共处理缓解了NaCl胁迫对加工番茄幼苗生长的抑制作用,使加工番茄幼苗仍保持较强的光合色素含量、较高的相对含水量和根系活力,降低了电解质渗出率、MDA含量、H_2O_2含量和O2-·产生速率,增强了加工番茄幼苗叶片中抗氧化酶SOD、POD和CAT活性,其中在2个品系中,50μmol/L H_2S和1.5 mmol/L H_2O_2共处理时可溶性糖分别比单独50μmol/L H_2S处理提高了24.0%和29.4%,脯氨酸含量分别提高了25.8%和21.3%,缓解盐胁迫效果最为显著。综上,外源H_2S和H_2O_2之间存在着一定程度的协同作用,可以诱导增强NaCl胁迫下加工番茄幼苗植株渗透调节能力、提高清除活性氧的酶促系统的防御能力,从而减弱加工番茄细胞内活性氧自由基对质膜的伤害,进而提高加工番茄幼苗对盐胁迫的适应性。其结果可为探讨H_2S和H_2O_2这2种信号分子协同作用、缓解加工番茄幼苗盐胁迫的生理机制提供理论依据。The effects of exogenous H2S and H2O2 physiological regulation on the processing tomato seedlings under NaCl stress were investigated,KT-32 with weak salt tolerance and KT-7 with strong salt tolerance as the materia. Research of 50 μmol/L H2S and H2O2 were determined by pot culture under the conditions of 0.5,1.5,3.0 mmol/L. Effects of cotreatment on the growth,osmoregulation,material accumulation and reactive oxygen metabolism of seedlings of processed tomato under 250 mmol/L NaCl stress.The results showed that the cotreatment of exogenous H2S and H 2O 2 alleviated the inhibitory effect of NaCl stress on the growth of seedlings of processed tomato seedlings,and maintained a strong photosynthetic pigment content,relatived water content and root activity in processed tomato seedlings.And decreased the electrolyte leakage rate,the content of MDA,the content of H 2O 2 and the production rate of O 2 -[]· free radical and enhanced the activities of antioxidant enzymes SOD,POD and CAT activity. Among the two strains,soluble sugar increased 24.0% and 29.4% by compared with 50 μmol/L H2S treatment alone at 50 μmol/L H2S and 1.5 mmol/L H2O2 ,proline content increased by 25.8% and 21.3%,respectively,and the effect of salt stress alleviation was the most significant.In summary,there were a certain degree of synergy between exogenous H2S and H2O2 ,which could enhance the osmotic regulatory capacity of plants under enhanced NaCl stress in tomato seedling plants to enhance the removal of reactive oxygen species H2S and H 2O 2 in the tomato seedlings,and then weakened the injury of reactive oxygen species on the plasma membrane in processed tomato cells and then increased the adaptability of tomato seedlings to salt stress. The synergistic effect of the two signaling molecules will provide the theoretical basis for alleviating the physiological mechanism of salt stress in tomato seedlings.

关 键 词：H2O2 H2S NACL胁迫 加工番茄幼苗 

分 类 号：S641.01[农业科学—蔬菜学]