基于席夫碱反应的氧化葡聚糖/胺化羧甲基壳聚糖双组分水凝胶粘合剂  

Oxidized dextran/aminated carboxymethyl chitosan two-component hydrogel adhesive based on Schiff base reaction

作　　者：李丹丹 莫秀梅[1] Li Dan-dan;Mo Xiu-mei(School of Chemical Engineering and Bioengineering,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海市201620

出　　处：《中国组织工程研究》2018年第22期3527-3532,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:基于席夫碱反应的双交联水凝胶粘合剂,具有一定的网络结构和更好的生物相容性,黏结强度也更高,在组织工程和临床医学中具有很好的应用前景。目的:通过席夫碱反应制备氧化葡聚糖/胺化羧甲基壳聚糖双组分水凝胶粘合剂,并进行表征。方法:利用高碘酸钠对葡聚糖进行氧化处理,利用乙二胺对羧甲基壳聚糖进行胺化处理;在室温条件下,通过席夫碱反应制备氧化葡聚糖/胺化羧甲基壳聚糖水凝胶粘合剂。利用红外吸收光谱表征氧化葡聚糖和胺化羧甲基壳聚糖的结构,利用分光光度计法测定氧化葡聚糖碘残留量,利用气相色谱法测定胺化羧甲基壳聚糖乙二胺残留量。根据行业标准,利用万能试验机检测氧化葡聚糖/羧甲基壳聚糖与氧化葡聚糖/胺化羧甲基壳聚糖水凝胶粘合剂的搭接-剪切拉伸承载强度、T-剥离拉伸承载强度、拉伸强度。结果与结论:(1)红外光谱显示,与葡聚糖相比,氧化葡聚糖的峰强度下降,同时在1 733 cm-1处出现一个新的吸收峰,对应于半缩醛结构;羧甲基壳聚糖本身具有胺基,在3 358 cm-1处宽峰对应于胺基与羟基的伸缩振动,胺化羧甲基壳聚糖在3 358 cm-1吸收峰大幅度增强;(2)氧化葡聚糖的氧化度为73.42%,碘残留量为138.58μg/g;胺化羧甲基壳聚糖胺基含量为0.636 9 mmol/L,无乙二胺残留;(3)氧化葡聚糖/胺化羧甲基壳聚糖水凝胶粘合剂的搭接-剪切拉伸承载强度、T-剥离拉伸承载强度和拉伸强度较氧化葡聚糖/羧甲基壳聚糖水凝胶粘合剂分别增大47.48%、17.54%和76.42%,增幅明显;(4)结果表明,氧化葡聚糖/胺化羧甲基壳聚糖水凝胶粘合剂具有较高的搭接-剪切拉伸承载强度、T-剥离拉伸承载强度和拉伸强度。BACKGROUND： Dual-crosslinked hydrogel adhesives based on Schiff base reaction have certain network structure, good biocompatibility and high bond strength, which have good application prospects in tissue engineering and clinical medicine.OBJECTIVE： To prepare and characterize oxidized dextran/aminated carboxymethyl chitosan two-component hydrogel adhesives through a Schiff base reaction. METHODS： Dextran was oxidized with sodium periodate and carboxymethyl chitosan was aminated with ethylenediamine. Oxidized dextran/aminated carboxymethyl chitosan hydrogel adhesives were prepared by Schiff base reaction at room temperature. The structures of oxidized dextran and aminated carboxymethyl chitosan were characterized by infrared absorption spectroscopy. The iodine residue of oxidized dextran was measured by spectrophotometer, and the ethylenediamine residue of aminated carboxymethyl chitosan was determined by gas chromatography. The lap-shear tensile load strength, T-peel tensile load strength and tensile strength of oxidized dextran/carboxymethyl chitosan and oxidized dextran/aminated carboxymethyl chitosan hydrogel adhesives were tested using a universal testing machine according to industry standards. RESULTS AND CONCLUSION：（1） IR spectra showed that the peak intensity of oxidized dextran decreased compared with that of dextran, with a new absorption peak appearing at 1 733 cm-1, corresponding to the hemiacetal structure. The carboxymethyl chitosan itself had broad peak at 3 358 cm-1, corresponding to the stretching vibration of the amine and hydroxyl groups, and the absorption peak at 3 358 cm-1 of aminated carboxymethyl chitosan increased significantly.（2） The oxidation degree of dextran was 73.42%, and the iodine residue was 138.58 μg/g. The amino group content of aminated carboxymethyl chitosan was 0.636 9 mmol/L and no ethylenediamine resided.（3） The lap-shear tensile load strength, T-peel tensile load strength and tensile strength of oxidized dextran/aminated carboxymethyl chitosan hydrog

关 键 词：葡聚糖类 壳聚糖 水凝胶 组织工程 生物材料 席夫碱 葡聚糖 羧甲基壳聚糖 黏结强度 

分 类 号：R318[医药卫生—生物医学工程]