电活性聚苯胺/聚乳酸支架的制备及生物相容性  

Preparation and biocompatibility of an electroactive polyaniline/poly(lactic-acid) scaffold

作　　者：王玲玲[1] 崔志明[1] 徐冠华[1] 李卫东[1] 保国锋[1] 孙郁雨[1] 陈佳佳[1] 张金龙[1] Wang Ling-ling;Cui Zhi-ming;Xu Guan-hua;Li Wei-dong;Bao Guo-feng;Sun Yu-yu;Chen Jia-jia;Zhang Jin-long(Department of Spinal Surgery,the Second Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China)

机构地区：[1]南通大学第二附属医院脊柱外科,江苏省南通市226001

出　　处：《中国组织工程研究》2018年第22期3557-3562,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省卫生科研项目(Z201316);江苏省六大人才高峰第十批次高层次人才资助项目(2013-WSW-007);江苏省中医药管理局科技项目(LB13020);南通市重大科技计划项目(HS2014002)~~

摘　　要：背景:有研究证实在电刺激条件下,聚苯胺/聚乳酸复合支架可促进神经干细胞突起的生长。目的:制备电活性聚苯胺/聚乳酸支架,观察其与大鼠嗅鞘细胞的生物相容性。方法:采用乳液聚合的方法制备聚苯胺/聚乳酸膜,电镜下观察其形态,测定其电导率。将SD乳鼠嗅鞘细胞分别接种于聚苯胺/聚乳酸膜上(实验组)与多聚赖氨酸包被的玻片上(对照组),进行细胞增殖、细胞黏附率测定及免疫荧光抗体标记观察。结果与结论:(1)扫描电镜下,聚苯胺/聚乳酸膜表面平整、均匀,可见为细小纤维形成的网格状结构,其电导率为1.5×10-5 S/cm;(2)培养1,3,5,7 d,实验组的细胞相对增殖率均在90%以上,细胞毒性为Ⅰ级;(3)培养7 d后,实验组细胞吸附率高于对照组(P<0.05);(4)培养7 d后免疫荧光检测显示,对照组S-100阳性细胞的数量及胞体面积与实验组无明显性差异(P>0.05),对照组S-100阳性细胞周长明显小于实验组(P<0.05);(5)结果表明,制备的聚苯胺/聚乳酸膜与嗅鞘细胞具有良好的生物相容性。BACKGROUND： Previous studies have confirmed that polyaniline/poly（lactic-acid） scaffold could promote the growth of neural stem cell processes in the condition of electrical stimulation.OBJECTIVE： To prepare an electroactive polyaniline/poly（lactic-acid） scaffold and to investigate its biocompatibility with rat olfactory ensheathing cells. METHODS： Olfactory ensheathing cells of Sprague-Dawley neonatal rats were inoculated onto the polyaniline/poly（lactic acid） film（experimental group） and poly lysine coating slides（control group）. Cell proliferation and cell adhesion rate were detected, and the cells were observed with immunofluorescence histochemistry. RESULTS AND CONCLUSION：（1） Smooth and even polyaniline/poly（lactic acid） film was visible under electron microscopy, with a grid structure formed by fine fibers, and its conductivity was 1.5×10-5 S/cm.（2） At 1, 3, 5, 7 days after co-culture, the cell relative growth rates were all above 90%, and the cytotoxicity was graded I.（3） At 7 days after co-culture, the cell adhesion rate in the experimental group was significantly higher than that in the control group（P〈0.05）.（4） At 7 days after co-culture, immunofluorescence results showed that there was no obvious difference between the two groups in the number or area of S-100 positive cells（P〉0.05）, while the cell perimeter in the experimental group was significantly higher than that in the control group（P〈0.05）. To conclude, the prepared polyaniline/poly（lactic acid） film has good biocompatibility with olfactory ensheathing cells.

关 键 词：生物相容性材料 脊髓损伤 组织工程 神经材料 导电生物材料 聚苯胺 聚乳酸 嗅鞘细胞 生物相容性 共培养 生物材料 

分 类 号：R318[医药卫生—生物医学工程]