5-氨基酮戊酸光动力疗法体外抑制断发毛癣菌肉芽肿株增殖研究  

In vitrostudy of the 5-aminolevulinic acid photodynamic therapy for Trichophyton tonsurans strain isolated from granuloma

作　　者：吴倩倩 石磊[2] 张海艳[2] 张玲琳[2] 高志琴 杨虹 杨连娟 王秀丽[1,2] WU Qian-qian 1 , SHI Lei 2 , ZHANG Hai-yan 2 , ZHANG Ling-lin 2 , GAO Zhi-qin 2 , YANG Hong 2 , YANG Lian-juan 2 , WANG Xiu-li 1,2(1. Shanghai Clinical College of Skin Disease, Anhui Medical University, Shanghai 200443, China;2. Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 20044)

机构地区：[1]安徽医科大学上海皮肤病临床学院,上海200443 [2]上海市皮肤病医院同济大学医学院,上海200443

出　　处：《中国真菌学杂志》2018年第3期139-143,共5页Chinese Journal of Mycology

基　　金：国家自然科学基金(81601601;81472538)

摘　　要：目的探讨5-氨基酮戊酸光动力疗法(5-Aminolevulinic acid photodynamic therapy,ALA-PDT)对体外培养的断发毛癣菌肉芽肿株的杀伤效应。方法将临床Majocchi肉芽肿患者皮损分离培养出的断发毛癣菌肉芽肿株,在马铃薯葡萄糖琼脂培养基上活化后制备菌悬液,与5mmol/L ALA共孵育后荧光显微镜观察原卟啉PpⅨ(ProtoporphyrinⅨ,PpⅨ)产生情况。实验分对照组、单药组、单光组和ALA-PDT组。对照组无处理。ALA-PDT组断发毛癣菌与5 mmol/L ALA共孵育6h后,采用功率密度为40mW/cm2的633nm红光以不同能量密度(50、100、150、175、200J/cm2)照射。单药组断发毛癣菌与5mmol/L ALA共孵育6h,不照光。单光组无ALA共孵育,只给予200J/cm2红光照射。通过数码相机拍摄照片及菌浓度评估ALA-PDT对断发毛癣菌肉芽肿株生长的抑制效应。结果荧光显微镜下可见明显砖红色荧光物质PpⅨ产生,当照光能量密度为175、200J/cm2时,照片显示菌落生长明显受限,数量减少,菌浓度计数分别为(0.53±0.058)×105 CFU/mL、(0.13±0.057)×105 CFU/mL,明显低于对照组、单药组、单光组及其他低能量密度ALA-PDT组(P<0.05)。结论 ALA-PDT对断发毛癣菌肉芽肿株有明确的杀伤效应,可为临床ALA-PDT治疗皮肤癣菌感染提供理论依据。Objective To demonstrate the fungicidal effect and action mechanism of 5-aminolevulinic acid photodynamic therapy（ALA-PDT）against Trichophyton tonsurans strain isolated from granuloma.Methods The clinical isolate of Trichophyton tonsurans granuloma was cultured in potato dextrose agar medium to prepare the suspension.After 6 hours of incubation,the production of PpⅨ was observed by fluorescence microscopy.Following PDT（at light energy density of 50,100,150,175,or 200 J/cm^2）,the growth inhibitory of this T.tonsurans strain was observed by colony cultivation.Results Fluorescence microscopy showed obvious PpⅨ production of brick red fluorescent substance.At the light energy density of175 and 200 J/cm^2,the significantly restricted growth of colonies was evidenced by decreased concentration of suspension.The suspension concentrations were（0.53±0.058）×10^5 CFU/mL and（0.13±0.057）×10^5 CFU/mL at light energy density of 175 and 200 J/cm^2 respectively,which were significantly lower than those of control group,single drug group,single light group and other low energy density ALA-PDT group（P〈0.05）.Conclusion ALA-PDT exerted fungicidal effect against Trichophyton tonsurans granuloma isolate,which might provide a theoretical basis for the clinical treatment of dermatophytes.

关 键 词：光动力治疗 断发毛癣菌 Majocchi肉芽肿 皮肤癣菌 

分 类 号：R379.2[医药卫生—病原生物学]