利福喷丁联合氟康唑对耐药白念珠菌细胞周期的影响  

The effect of rifapentine and fluconazole on the cell cycle of FLC-resistance Candida albicans

作　　者：王玉连[1] 袁公 陈雅楠 曹永兵[2] 吴建华[1] WANG Yu-lian 1 ,YUAN-Gong 1 ,CHEN Ya-nan 1 ,CAO Yong-bing 2 ,WU Jian-hua 1(1.Department of Dermatology, Changhai Hospital, Second Military Medical University,Shanghai 200433, China; 2.New Drug Research and Development Center, School of Pharmacology, Second Military Medical University, Shanghai 200433, China)

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]第二军医大学药学院新药研究中心,上海200433

出　　处：《中国真菌学杂志》2018年第3期144-147,共4页Chinese Journal of Mycology

基　　金：国家973项目子课题(2013CB531602)

摘　　要：目的探索利福喷丁(rifapentine)联合氟康唑(fluconazole)对耐氟康唑白念珠菌细胞周期的影响。方法将白念珠菌菌悬液与利福喷丁和(或)氟康唑共同培养12h,碘化丙啶(PI)染色后流式细胞仪检测空白对照组、利福喷丁组、氟康唑组及利福喷丁与氟康唑联合用药组DNA含量,分析利福喷丁及其联合氟康唑对细胞周期的影响。结果与空白对照组、利福喷丁组及氟康唑组相比,联合用药组G0/G1期DNA含量均减少(P<0.01),G2/M期DNA含量均显著增加(P<0.01)。说明利福喷丁与氟康唑合用时组细胞周期阻滞在G2/M期。结论利福喷丁与氟康唑联合用药时能阻断耐药白念珠菌细胞周期进程,抑制细胞的正常分裂增殖。Objective To explore the effect of rifapentine and flueonazole on the cell cycle of FLC-resistance Candida albicans in vitro.Methods Candida albicans suspension were cocultured with rifapentine alone or combined with flueonazole for12 h.A blank control group without drugs was established.The DNA content of Candida albicans in each group was detected by Flow Cytometrya after Propidium iodide（PI）staining.The effects of drugs on cell cycle of FLC-resistance Candida albicans including the rifapentine alone or combined with flueonazole were analyzed.Results Compared with growth control group and single drug group,DNA content decreased in G0/G1 phase（P〈0.01）of combination group,but significantly increased in G2/M phase（P〈0.01）.This showed that cell cycle was inhibited at G2/M phase when rifapentine and fluconazole were conbined.Conclusion The results showed that the combination of rifapentine and fluconazole had a significantly effect on arresting of FLC-resistance Candida albicans＇ cell cycel and inhibiting its normal division and proliferation.

关 键 词：白念珠菌 利福喷丁 氟康唑 细胞周期 

分 类 号：R379.4[医药卫生—病原生物学] R978.5[医药卫生—基础医学]