大麦TILLING群体的构建及EDR1基因突变的检测(英文)  

TILLING-population Analysis of EDR1 in Barley Cultivar 'Xiyin2'

作　　者：吕瑞华 冯昭 冯飞雪 陈耀锋[3] LU Ruihua;FENG Zhao;FENG Feixue;CHEN Yaofeng(College of Medical Technology,Shaanxi University of Chinese Medicine,Xianyang,Shaanxi 712046,China;Department of Clinical Laboratory,Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang,Shaanxi 712000,China;College of Agronomy,Northwest A＆F University,Yangling,Shaanxi 712100,China)

机构地区：[1]陕西中医药大学医学技术学院,陕西咸阳712046 [2]陕西中医药大学附属医院检验科,陕西咸阳712000 [3]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2018年第6期1024-1031,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西省科技统筹创新工程计划(2015KTCL02-24)

摘　　要：该研究选用栽培大麦品种‘西引2号’,通过化学诱变剂甲基磺酸乙酯(ethyl methane sulfonate,EMS)处理,创建了3 750个M1及2 012个M2突变株系。结果表明:在M2中,有115株表现黄化,98株表现矮化,101株表现极早或极晚抽穗,其他35株表现分蘖少、叶片小或者不育等性状,所有突变株系占M2群体的17.35%;M2的基因组DNA用于突变频率的研究,用优化的CELⅠ酶切体系对M2株系进行了EDR1基因突变体筛选,获得了EDR1基因的3个突变体,其中一个发生在外显子区域,导致了氨基酸Pro到Ser的转换,突变频率平均为每661kb一个点突变,这与前人研究的突变频率基本在一个范围内。该研究创建了大麦品种‘西引2号’的TILLING筛选群体,并用于EDR1突变体的筛选。为大麦性状研究提供了不同的思路,搭建了新的资源和技术平台。To advance barley research,we produced a TILLING（Targeting Induced Local Lesions IN Genomes）population of the six-rowed barley cultivar‘Xiyin2＇.Through ethyl methane sulfonate（EMS）mutagenesis treatment,we established a 3 750 mutagenised M1 population and a 2 012 mutagenised M2 population.Totally phenotypic variations in 17.35% of the M2 population were observed.In which 115 lines showed chlorosis,98 lines showed dwarfing,101 lines showed very early/late flowering,and others 35 lines showed less tilling,small leaves,or produced no seeds.Likewise,genomic DNA was extracted from the M2 lines for studying the mutation frequency.The estimated mutation frequency was one mutation per661 kb by CELⅠ-based screening,it is the same range as the previously studies.During our investigation,3 different mutations in the EDR1（AF305912）gene were identified,among which the Pro to Ser transition happened in extron region.The‘Xiyin2＇TILLING population will be an important resource in the development of barley with specific characters.

关 键 词：大麦 EDR1基因 TILLING技术 

分 类 号：Q319.2[生物学—遗传学]