36个葡萄品种遗传多样性ISSR分析  

Genetic diversity of 36 grape germplasms revealed by ISSR markers

作　　者：吴代东[1] 陈立[2] 李洪艳[1] 吴艳艳[3] 周咏梅[1] 夏周祥 谢太理[1] WU Daidong1,CHEN Li2,LI Hongyan1,WU Yanyan3,ZHOU Yongmei1,XIA Zhouxiang4,XIE Taili1(1.Viticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences,Nanning 530007;2.National Demonstration Center for Experimental Plant Science Education （Guangxi University）,Nanning 530004,China;3.Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;4.College of Life Sciences, Guangxi University,Nanning 530004,China)

机构地区：[1]广西农业科学院葡萄与葡萄酒研究所,南宁530007 [2]广西大学植物科学国家级实验教学示范中心,南宁530004 [3]广西农业科学院生物技术研究所,南宁530007 [4]广西大学生命科学院,南宁530004

出　　处：《中外葡萄与葡萄酒》2018年第4期34-37,共4页Sino-Overseas Grapevine & Wine

基　　金：国家农业产业技术体系广西特色水果创新团队(nycytxgxcxtd-04-19-14);桂南桂西葡萄育种栽培岗位(nycytxgxcxtd-04-19-11);桂科转14125003-1-15;广西罗城毛葡萄试验站[桂政办发(2014)99号]

摘　　要：本研究探究了36份抗病性不同的葡萄种质,构建了不同种之间的遗传关系图谱,为葡萄的选育及其栽培提供一定的依据。提取36份葡萄材料总DNA,并通过ISSR标记筛选出两条多态性丰富的引物,通过非加权配对算术平均法(UPGMA)对ISSR-PCR得到的不同葡萄品种进行聚类分析,建立亲缘关系图。筛选得到的序列ISSR820对36份材料PCR扩增得到不同片段的DNA条带多达208条,多态性比率高达83%。ISSR分子标记将36个葡萄品种主要分为两大类。36 species of grape were explored highly resistant wild grape resources and constructed genetic relationships. The results laid the foundation for the breeding and cultivation of grape in agriculture. Totals DNA of 36 grape materials were extracted and amplified two polymorphic primers through ISSR markers. We conducted cluster analysis of different grape varieties obtained by ISSR-PCR through unweighted paired arithmetic mean（UPGMA） and established the relationship diagram. The result showed that the ISSR820 sequences screened with better polymorphism. DNA bands of different fragments were amplified by PCR of 36 materials for this sequence, up to 208. The polymorphism rate was as high as 83%. The ISSR molecular markers could be used to mainly divide the 36 species of grape into two clusters.

关 键 词：ISSR 葡萄 亲缘关系 遗传 

分 类 号：S663.1[农业科学—果树学] Q75[农业科学—园艺学]