青花菜黑腐病致病菌的分离和鉴定  

Isolation and identification of the bacterial pathogen causing black rot in broccoli

作　　者：刘莉莉[1] 文正华[1] 单晓政[1] 张文慧 贺丽霞 宋文芹[2] 孙德岭[1] 江汉民[1] LIULili1,WENZhenghua1,SHANXiaozheng1,ZHANGWenhui1, 2,HELixia1, 2,SONGWenqin2,SUNDeling1,JIANGHanmin1(1.Key Laboratory of Vegetable Germplasm Resources Innovation,Key Laboratory of Tianjin Vegetable Genetic Breeding Enterprise,Tianjin Kernel Vegetable Research Institute,Tianjin 300381,China; 2.College of LifeSciences, Nankai University,Tianjin 300381,Chin)

机构地区：[1]蔬菜种质创新国家重点实验室.天津市蔬菜遗传育种企业重点实验室.天津科润蔬菜研究所,天津300384 [2]南开大学生命科学学院,天津300371

出　　处：《中国瓜菜》2018年第7期18-22,共5页China Cucurbits And Vegetables

基　　金：国家大宗蔬菜产业技术体系专项(CARS-25-A-13);国家重点研发计划子项目"花椰菜;青花菜优质多抗适用性强新品种培育"(2017YFD0101805);天津市青年科研人员创新研究与实验项目(2018002)

摘　　要：黑腐病是影响青花菜生产的主要病害之一,为了更好地研究该致病菌的致病机制和十字花科蔬菜对其抗性的分子机制,从发病田间采集具有典型症状的病害标样,对病原菌进行分离、纯化,并对其形态特征、理化特性和致病性进行了分析,同时测定了该菌株23S rDNA和hrcC基因序列,分析了与近缘细菌23S rDNA和hrcC基因序列的同源性,构建了系统发生树,比较了2种基因对相似细菌的检测和鉴别能力,结果表明,hrcC基因用于该致病菌的鉴定更具优越性。根据分离菌的表型特征及分子特征,判定该分离菌为青花菜黑腐病的致病菌-野油菜黄单胞杆菌野油菜致病变种。The black rot caused by Xanthomonas campestris pv. Campestris is one of the main disease affecting broccoli production. In order to study the pathogenic mechanism of the pathogenic bacteria and the molecular mechanism of crucifer resistance to the pathogen, diseased samples with typical symptom were collected and representative strains were selected to determine morphology, cultural characters, straining reaction, physiological and biochemical reactions and pathogenicity of pathogen. In addition, the 23S rDNA and hrcC gene were partially sequenced and compared with the sequences deposited in databased, molecular phylogentic trees were constructed based on two genes. The results showed that hrcC gene analysis may be a more useful tool for interspecies identification. The isolates were identified as Xanthomonas campestris pv. Campestris based on their phenotepic and molecular characteristics.

关 键 词：青花菜 黑腐病 23S RDNA hrcC基因 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]